Journal of Hepatology
Volume 41, Issue 1 , Pages 82-88 , July 2004

Impairment of mitochondrial oxidative phosphorylation in rat fatty liver exposed to preservation-reperfusion injury

  • Paolo Caraceni

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • Centro Ricerca Biomedica Applicata (C.R.B.A.), University of Bologna, 40138 Bologna, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39-51-636-4912; fax: +39-51-340-877
    • ,
  • Cristina Bianchi

      Affiliations

    • Dipartimento di Biochimica, University of Bologna, 40138 Bologna, Italy
    • ,
  • Marco Domenicali

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • Centro Ricerca Biomedica Applicata (C.R.B.A.), University of Bologna, 40138 Bologna, Italy
    • ,
  • Anna Maria Pertosa

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • Centro Ricerca Biomedica Applicata (C.R.B.A.), University of Bologna, 40138 Bologna, Italy
    • ,
  • Elisabetta Maiolini

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • ,
  • Giovanna Parenti Castelli

      Affiliations

    • Dipartimento di Biochimica, University of Bologna, 40138 Bologna, Italy
    • ,
  • Bruno Nardo

      Affiliations

    • Centro Ricerca Biomedica Applicata (C.R.B.A.), University of Bologna, 40138 Bologna, Italy
    • Dipartimento di Scienze Chirurgiche, Rianimatorie e dei Trapianti, University of Bologna, 40138 Bologna, Italy
    • ,
  • Franco Trevisani

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • ,
  • Giorgio Lenaz

      Affiliations

    • Dipartimento di Biochimica, University of Bologna, 40138 Bologna, Italy
    • ,
  • Mauro Bernardi

      Affiliations

    • Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, Policlinico Sant'Orsola, Via Massarenti 9, 40138 Bologna, Italy
    • Centro Ricerca Biomedica Applicata (C.R.B.A.), University of Bologna, 40138 Bologna, Italy

Received 14 July 2003 ,Revised 12 February 2004 ,Accepted 31 March 2004.

References 

  1. Trevisani F, Colantoni A, Caraceni P, Van Thiel DH. The use of donor fatty liver for liver transplantation: a challenge or a quagmire?. J Hepatol. 1996;24:114–121
  2. Selzner M, Clavien PA. Fatty liver in liver transplantation and surgery. Semin Liver Dis. 2001;21:105–113
  3. Vajdovà K, Graf R, Clavien PA. ATP-supplies in the cold preserved livers: a long-neglected factor of organ viability. Hepatology. 2002;36:1543–1552
  4. Lenaz G. Role of mitochondria in oxidative stress and ageing. Biochim Biophys Acta. 1998;1366:53–67
  5. Rosser BG, Gores GJ. Liver cell necrosis: cellular mechanism and clinical implication. Gastroenterology. 1995;108:252–275
  6. Hui A, Kawasaki S, Makuuchi M, Nakayama J, Ikegami T, Miyagawa J. Liver injury following normothermic ischemia in steatotic rat liver. Hepatology. 1994;20:1287–1293
  7. Fukumori T, Ohkohchi N, Tsukamoto S, Satomi S. Why is fatty liver unsuitable for transplantation? Deterioration of mitochondrial ATP synthesis and sinusoidal structure during cold preservation of a liver with steatosis. Transplant Proc. 1997;29:412–415
  8. Miki C, Iriyama K, Mirza DF, Mayer AD, Buckels JA, Suzuki H, et al.  Postperfusion energy metabolism of steatotic graft and its relation to early graft viability following liver transplantation. Dig Dis Sci. 1998;43:74–79
  9. Hakamada K, Sasaki M, Takahashi K, Umehara Y, Konn M. Sinusoidal flow block after warm ischemia in rats with diet-induced fatty liver. J Surg Res. 1997;70:12–20
  10. Vendemiale G, Grattagliano I, Caraceni P, Caraccio G, Domenicali M, Dall'Agata M, et al.  Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status. Hepatology. 2001;33:808–815
  11. Ghoshal AK, Farber E. Choline deficiency, lipotrope deficiency and the development of liver disease including liver cancer: a new perspective. Lab Invest. 1993;68:255–260
  12. Caldwell SH, Swerdlow RH, Khan EM, Iezzoni JC, Hespenheide EE, Parks JK, et al.  Mitochondrial abnormalities in non-alcoholic stetatohepatitis. J Hepatol. 1999;31:430–434
  13. Rashid A, Wu TC, Huang CC, Chen CH, Lin HZ, Yang SQ, et al.  Mitochondrial proteins that regulate apoptosis and necrosis are induced in mouse fatty liver. Hepatology. 1999;29:1131–1138
  14. Hayashi M, Tokunaga Y, Fujita T, Tanaka K, Yamaoka Y, Ozawa K. The effects of cold preservation on steatotic graft viability in rat liver transplantation. Transplantation. 1993;56:282–287
  15. Minor T, Akbar S, Tolba R, Dombrowski F. Cold preservation of fatty liver grafts: pre16 vention of functional and ultrastructural impairments by venous oxygen persufflation. J Hepatol. 2000;32:105–111
  16. Caraceni P, Nardo B, Domenicali M, Turi P, Vici M, Simoncicni M, et al.  Ischemia-reperfusion injury in the rat fatty liver: role of nutritional status. Hepatology. 1999;29:1139–1146
  17. Hoyumpa AM, Greene HL, Dunn GD, Schenker SDD. Fatty liver: biochemical and clinical con18 siderations. Dig Dis. 1975;20:1142–1149
  18. Kim SK, Belzer FO, Southard JH. Loss of mitochondrial respiratory function and its suppression during cold ischemic preservation of rat livers with University of Wisconsis solution. Hepatology. 1992;16:742–748
  19. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949;177:752–766
  20. Chance B. In:  Wolstenholme GE,  O'Conner CM editor. Ciba foundation symposium on regulation of cell metabolism. London: J & A Churcill Ltd; 1959;p. 91–129
  21. Estabrook RW. Mitochondrial respirarory control and the polarographic measurement of ADP:O ratios. Methods Enzymol. 1967;10:41–47
  22. Heytler PG. Uncouplers of oxidative phosphorylation. Methods Enzymol. 1979;60:462–472
  23. Slater EC. Application of inhibitor and uncouplers for a study of oxidative phosphorylation. Methods Enzymol. 1967;10:48–57
  24. Castelluccio C, Baracca A, Fato R, Pallotti F, Maranesi M, Barzanti V, et al.  Mitochondrial activities of rat heart during aging. Mech Ageing Dev. 1994;76:73–88
  25. Barogi S, Baracca A, Cavazzoni M, Parenti Castelli G, Lenaz G. Effect of the oxidative stress induced by adriamycin on rat hepatocyte bioenergetics during ageing. Mech Ageing Dev. 2000;113:1–21
  26. Jaworec D, Greber W, Bergmeyer HV. Adenosine-5′-triphosphate. In:  Bergmeyer HV editors. Methods of enzymatic analysis. 2nd ed.. New York, NY: Academic Press; 1974;p. 2097–2101
  27. Sammut IA, Thorniley MS, Simpkin S, Fuller BJ, Bates TE, Green CJ. Impairment of mitochondrial respiratory function following storage and orthotopic transplantation of rat livers. Cryobiology. 1998;36:49–60
  28. Tsunekawa S, Tanaka A, Ozawa K. Molecular damage to rat liver mitochondrial (H+)-ATPase during cold preservation with UW solution. Transplantation. 1991;52:999–1003
  29. Hensley K, Kotake K, Sang H, Pye QN, Wallis GJ, Kolker LM, et al.  Dietary choline restriction causes complex I dysfunction and increased H2O2 generation in liver mitochondria. Carcinogenesis. 2000;21:983–989
  30. Lieber CS. Biochemical and molecular basis of alcoholic-induced injury to liver and other tissues. N Engl J Med. 1988;319:1639–1650
  31. Taylor WS, Fahy E, Murray J, Capaldi RA, Ghosh SS. Oxidative post-translational modification of tryptophan residues in cardiac mitochondrial proteins. J Biol Chem. 2003;278:19587–19590

PII: S0168-8278(04)00158-8

doi: 10.1016/j.jhep.2004.03.022

Journal of Hepatology
Volume 41, Issue 1 , Pages 82-88 , July 2004

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