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The protective role of hydrogen-rich saline in experimental liver injury in mice

  • Author Footnotes
    § These authors contributed equally to this work.
    HanYong Sun
    Footnotes
    § These authors contributed equally to this work.
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China

    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • Author Footnotes
    § These authors contributed equally to this work.
    Lei Chen
    Footnotes
    § These authors contributed equally to this work.
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China
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  • Author Footnotes
    § These authors contributed equally to this work.
    WeiPing Zhou
    Footnotes
    § These authors contributed equally to this work.
    Affiliations
    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • Liang Hu
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China
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  • Liang Li
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China
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  • QianQian Tu
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China

    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • YanXin Chang
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China

    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • Qu Liu
    Affiliations
    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • XueJun Sun
    Affiliations
    Department of Diving Medicine, Second Military Medical University, Shanghai 200433, PR China
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  • MengChao Wu
    Affiliations
    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China
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  • HongYang Wang
    Correspondence
    Corresponding author at: International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China. Tel.: +86 21 81875361; fax: +86 21 65566851.
    Affiliations
    International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, PR China

    Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China

    National Laboratory for Oncogenes and Related Genes, Cancer Institute in RenJi Hospital, Shanghai Jiao Tong University, Shanghai 200441, PR China
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  • Author Footnotes
    § These authors contributed equally to this work.
Published:November 03, 2010DOI:https://doi.org/10.1016/j.jhep.2010.08.011

      Background & Aims

      Reactive oxygen species (ROS) are considered to play a prominent causative role in the development of various hepatic disorders. Antioxidants have been effectively demonstrated to protect against hepatic damage. Hydrogen (H2), a new antioxidant, was reported to selectively reduce the strongest oxidants, such as hydroxyl radicals (OH) and peroxynitrite (ONOO), without disturbing metabolic oxidation–reduction reactions or disrupting ROS involved in cell signaling. In place of H2 gas, hydrogen-rich saline (HS) may be more suitable for clinical application. We herein aim to verify its protective effects in experimental models of liver injury.

      Methods

      H2 concentration in vivo was detected by hydrogen microelectrode for the first time. Liver damage, ROS accumulation, cytokine levels, and apoptotic protein expression were, respectively, evaluated after GalN/LPS, CCl4, and DEN challenge. Simultaneously, CCl4-induced hepatic cirrhosis and DEN-induced hepatocyte proliferation were measured.

      Results

      HS significantly increased hydrogen concentration in liver and kidney tissues. As a result, acute liver injury, hepatic cirrhosis, and hepatocyte proliferation were reduced through the quenching of detrimental ROS. Activity of pro-apoptotic players, such as JNK and caspase-3, were also inhibited.

      Conclusions

      HS could protect against liver injury and also inhibit the processes leading to liver cirrhosis and hepatocyte compensatory proliferation.

      Abbreviations:

      ROS (reactive oxygen species), OH (hydroxyl radicals), ONOO− (peroxynitrite), HS (hydrogen-rich saline), NS (normal saline), GalN (d-galactosamine), LPS (lipopolysaccharide), CCl4 (carbon tetrachloride), DEN (diethylnitrosamine), AHF (acute hepatic failure), H2 (hydrogen), O2-· (superoxide anion radical), H2O2 (hydrogen peroxide), NO (nitric oxide), IHC (immunohistochemical), DHE (dihydroethidine), HSC (hepatic stellate cells), RC (regular chow)

      Keywords

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