Abstract
Background/Aims: The mechanisms underlying alcohol-induced liver injury are not fully elucidated.
An approach in this direction would consist of an all-inclusive assessment of gene
expression in the liver. The purpose of this study was to perform a comprehensive
analysis of gene expression in the livers of mice treated with ethanol by means of
intragastric infusion.
Methods: An ethanol- or glucose-enriched liquid diet was fed to animals for 4 weeks via a
long-term gastrostomy catheter. The animals were killed and plasma alanine:2-oxoglutarate
aminotransferase (ALT) assay, liver histology and total RNA analysis by microarray
gene technology were performed.
Results: Alcohol increased ALT, induced steatosis, necrosis and inflammation. A total of
12,423 genes were analyzed for expression out of which 4867 were expressed by the
liver. Alcohol repressed expression of 11 genes, induced expression of 13 genes, and
up- or down-regulated expression of 44 and 42 genes >2-fold, respectively. Gene expression
analysis identified several genes that have not previously been tested for alcohol
effects.
Conclusions: This study: (i) expands the knowledge of mechanism(s) of action of ethanol; (ii)
indicates novel pathways of ethanol action on the liver, and (iii) illustrates the
utility of microarray gene analysis in hepatology research.
Keywords
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Article info
Publication history
Accepted:
October 16,
2003
Received in revised form:
October 6,
2003
Received:
March 19,
2003
MS 287Identification
Copyright
© 2003 European Association for the Study of the Liver. Published by Elsevier Inc. All rights reserved.
