Abstract
Background/Aims: To clarify the association between the reovirus infection of the hepatobiliary tree
and the development of infantile obstructive cholangiopathy (IOC) including biliary
atresia (BA) and congenital dilatation of the bile duct (CBD).
Methods: We designed reovirus common primers for nested RT-PCR based on the L3 gene segment.
The spectrum and the sensitivity of common primers were evaluated with purified reoviral
RNAs and reovirus mixed with stool samples. Then, nested RT-PCRs were performed with
hepatobiliary and fecal samples obtained from patients with BA, CBD, and control diseases.
Additionally, electron microscopy of stool samples was performed.
Results: The L3 common primers could amplify cDNAs synthesized from RNAs of three prototypes
of reovirus, and detect as much as 5.0×103 plaque forming unit of serotype 3 Dearing strain in 100 mg of fecal samples. However,
no amplification product was detected in 136 hepatobiliary tissues taken from 67 patients
including 26 BAs and 28 CBDs, or in 65 fecal samples obtained from 15 patients including
10 BAs and 1 CBD. Additionally, viral particles were not found in any stool specimens
by the electron microscope.
Conclusions: These data do not suggest that reoviruses play a major role in the etiology of IOC
or BA.
Keywords
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Article info
Publication history
Accepted:
October 31,
2003
Received in revised form:
October 14,
2003
Received:
May 2,
2003
MS 438Identification
Copyright
© 2003 Published by Elsevier Inc.
