Background/Aims
The aim of this study was to investigate the effect and the mechanism of hyperbaric
oxygen treatment on regenerating rat liver after partial hepatectomy (PH).
Methods
Wistar rats underwent a 70% PH, followed by treatment with hyperbaric oxygen starting
8 h after PH. The regenerated liver weight and serum parameters were compared. Proliferation
of both hepatocytes and sinusoidal endothelial cell (SEC) was also monitored by evaluating
the proliferating cell nuclear antigen (PCNA) labeling index. Furthermore, the hepatic
adenosine triphosphate levels and vascular endothelial growth factor (VEGF) protein
expression were analyzed at different times.
Results
Hyperbaric oxygen treatment significantly reduced the serum alanine aminotransferase
levels at 24 h, total bilirubin and total bile acid levels at 48 and 72 h, respectively. No significant differences in the hepatic adenosine triphosphate
levels, the restitution of liver weight, or PCNA positive hepatocytes were observed
between the two groups. The PCNA positive SEC, in contrast, was significantly increased
in the hyperbaric oxygen group at 48 h, furthermore, the hyperbaric oxygen treatment significantly increased the expression
of VEGF protein in the regenerating liver at 24 and 48 h.
Conclusions
Hyperbaric oxygen treatment can be considered as a therapeutic modality after massive
PH.
Keywords
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Article info
Publication history
Published online: February 03, 2006
Accepted:
December 15,
2005
Received in revised form:
December 13,
2005
Received:
October 7,
2005
Identification
Copyright
© 2006 European Association for the Study of the Liver. Published by Elsevier Inc. All rights reserved.
