Background/Aims
To evaluate feasibility, safety and pattern of bone marrow-derived cells (BMC) mobilization
in patients with end stage liver cirrhosis following granulocyte-colony stimulating
factor (G-CSF) administration.
Methods
Eight patients with severe liver cirrhosis (Child–Pugh score B-C, spleen diameter
less than 170 mm) were included. They were treated with G-CSF (5 μg/kg b.i.d for three consecutive days) to mobilize BMC, evaluated as circulating
CD34+ve cells (flow cytometry) and myeloid CFU-GM progenitors (in vitro colony growth
assay). Co-expression in CD34+ve cells markers of differentiation (Thy1, CD133, CXCR4,
c1qRp) were investigated on CD34+ve cells by double direct immunofluorescence. Data
from 40 healthy haematopoietic stem cell donors were used as controls.
Results
Mobilization of CD34+ve cells occurred in all patients. It was paralleled by expansion
of circulating CFU-GM progenitors. Circulating CD34+ve cells co-expressed epithelial
and stem cell markers in both cirrhotics and volunteer stem cell donors. G-CSF was
well tolerated, no adverse event occurred, a significant reversible increase of splenic
longitudinal diameter was observed.
Conclusions
(i) G-CSF mobilization of BMC co-expressing epithelial and stem markers occurred in
all cirrhotic patients; (ii) splenomegaly up to 170 mm does not prevent safe BMC mobilization following G-CSF in patients with end stage
liver disease; (iii) mobilized BMC may represent an easy immature cell source potentially
useful for novel approaches for liver regeneration.
Keywords
Abbreviations:
BMC (bone marrow-derived cells), G-CSF (granulocyte-colony stimulating factor), PB (peripheral blood), US (ultrasound), WBC (white blood cell)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: April 06, 2006
Accepted:
February 6,
2006
Received in revised form:
January 20,
2006
Received:
November 9,
2005
Footnotes
☆The authors who have taken part in this study declared that they have no relationship with the manufacturers of the drugs involved either in the past or present and did not receive funding from the manufacturers to carry out their research and from any source to carry out this study.
Identification
Copyright
© 2006 European Association for the Study of the Liver. Published by Elsevier Inc. All rights reserved.
