Advertisement

Hepatic Encephalopathy in Chronic Liver Disease: 2014 Practice Guideline by the European Association for the Study of the Liver and the American Association for the Study of Liver Diseases

  • American Association for the Study of Liver Diseases
    Author Footnotes
    Contributors: Chairman: Hendrik Vilstrup. Clinical Practice Guideline Members: Piero Amodio, Jasmohan Bajaj, Juan Cordoba (Deceased) , Peter Ferenci, Kevin D. Mullen, Karin Weissenborn, Philip Wong. AASLD/EASL Practice Guideline Subcommittee on Hepatic Encephalopathy: Jayant A. Talwalkar (Chair, AASLD), Hari S. Conjeevaram, Michael Porayko, Raphael B. Merriman, Peter L.M. Jansen, Fabien Zoulim.This guideline has been approved by the American Association for the Study of Liver Diseases and the European Association for the Study of the Liver and represents the position of both associations.These guidelines were developed by AASLD and EASL and are published simultaneously in Hepatology (volume 60, issue 2) and the Journal of Hepatology (volume 61, issue 3).
  • European Association for the Study of the Liver
    Author Footnotes
    Contributors: Chairman: Hendrik Vilstrup. Clinical Practice Guideline Members: Piero Amodio, Jasmohan Bajaj, Juan Cordoba (Deceased) , Peter Ferenci, Kevin D. Mullen, Karin Weissenborn, Philip Wong. AASLD/EASL Practice Guideline Subcommittee on Hepatic Encephalopathy: Jayant A. Talwalkar (Chair, AASLD), Hari S. Conjeevaram, Michael Porayko, Raphael B. Merriman, Peter L.M. Jansen, Fabien Zoulim.This guideline has been approved by the American Association for the Study of Liver Diseases and the European Association for the Study of the Liver and represents the position of both associations.These guidelines were developed by AASLD and EASL and are published simultaneously in Hepatology (volume 60, issue 2) and the Journal of Hepatology (volume 61, issue 3).
  • Author Footnotes
    Contributors: Chairman: Hendrik Vilstrup. Clinical Practice Guideline Members: Piero Amodio, Jasmohan Bajaj, Juan Cordoba (Deceased) , Peter Ferenci, Kevin D. Mullen, Karin Weissenborn, Philip Wong. AASLD/EASL Practice Guideline Subcommittee on Hepatic Encephalopathy: Jayant A. Talwalkar (Chair, AASLD), Hari S. Conjeevaram, Michael Porayko, Raphael B. Merriman, Peter L.M. Jansen, Fabien Zoulim.This guideline has been approved by the American Association for the Study of Liver Diseases and the European Association for the Study of the Liver and represents the position of both associations.These guidelines were developed by AASLD and EASL and are published simultaneously in Hepatology (volume 60, issue 2) and the Journal of Hepatology (volume 61, issue 3).
Open AccessPublished:July 08, 2014DOI:https://doi.org/10.1016/j.jhep.2014.05.042

      Abbreviations:

      AASLD (American Association for the Study of Liver Diseases), ACLF (acute-on-chronic liver failure), ALD (alcoholic liver disease), ALF (acute liver failure), BCAAs (branched-chain amino acids), CFF (Critical Flicker Frequency), CHE (covert HE), CLD (chronic liver disease), CRT (Continuous Reaction Time), CT (computed tomography), DM (diabetes mellitus), EASL (European Association for the Study of the Liver), EEG (electroencephalography), GI (gastrointestinal), GRADE (the Grading of Recommendation Assessment, Development, and Evaluation), GCS (Glasgow Coma Scale), GPB (glyceryl phenylbutyrate), HCV (hepatitis C virus), HE (hepatic encephalopathy), HM (hepatic myelopathy), ICT (Inhibitory Control Test), ISHEN (International Society for Hepatic Encephalopathies and Nitrogen Metabolism), IV (intravenous), LOLA (L-ornithine L-aspartate), LT (Liver transplantation), MHE (minimal HE), MR (magnetic resonance), OHE (overt HE), PH (portal hypertension), PHES (Psychometric Hepatic Encephalopathy Score), PP (portal pressure), PSE (portosystemic encephalopathy), PSS (portosystemic shunting), RCT (randomized, controlled trial), TIPS (transjugular intrahepatic portosystemic shunt), VB (variceal bleeding), WHC (West Haven Criteria), WM (working memory)

      Preamble

      These recommendations provide a data-supported approach. They are based on the following: (1) formal review and analysis of the recently published world literature on the topic; (2) guideline policies covered by the American Association for the Study of Liver Diseases/European Association for the Study of the Liver (AASLD/EASL) Policy on the Joint Development and Use of Practice Guidelines; and (3) the experience of the authors in the specified topic.
      Intended for use by physicians, these recommendations suggest preferred approaches to the diagnostic, therapeutic, and preventive aspects of care. They are intended to be flexible, in contrast to standards of care, which are inflexible policies to be followed in every case. Specific recommendations are based on relevant published information.
      To more fully characterize the available evidence supporting the recommendations, the AASLD/EASL Practice Guidelines Subcommittee has adopted the classification used by the Grading of Recommendation Assessment, Development, and Evaluation (GRADE) workgroup, with minor modifications (Table 1). The classifications and recommendations are based on three categories: the source of evidence in levels I through III; the quality of evidence designated by high (A), moderate (B), or low quality (C); and the strength of recommendations classified as strong (1) or weak (2).
      Table 1GRADE system for evidence.

      Literature review and analysis

      The literature databases and search strategies are outlined below. The resulting literature database was available to all members of the writing group (i.e., the authors). They selected references within their field of expertise and experience and graded the references according to the GRADE system [
      • Guyatt G.H.
      • Oxman A.D.
      • Vist G.E.
      • Kunz R.
      • Falck-Ytter Y.
      • Alono-Coello P.
      • et al.
      GRADE: an emerging consensus on rating quality of evidence and strength of recommendations.
      ]. The selection of references for the guideline was based on a validation of the appropriateness of the study design for the stated purpose, a relevant number of patients under study, and confidence in the participating centers and authors. References on original data were preferred and those that were found unsatisfactory in any of these respects were excluded from further evaluation. There may be limitations in this approach when recommendations are needed on rare problems or problems on which scant original data are available. In such cases, it may be necessary to rely on less-qualified references with a low grading. As a result of the important changes in the treatment of complications of cirrhosis (renal failure, infections, and variceal bleeding [VB]), studies performed more than 30 years ago have generally not been considered for these guidelines.

      Introduction

      Hepatic encephalopathy (HE) is a frequent complication and one of the most debilitating manifestations of liver disease, severely affecting the lives of patients and their caregivers. Furthermore, cognitive impairment associated with cirrhosis results in utilization of more health care resources in adults than other manifestations of liver disease [
      • Rakoski M.O.
      • McCammon R.J.
      • Piette J.D.
      • Iwashyna T.J.
      • Marrero J.A.
      • Lok A.S.
      • et al.
      Burden of cirrhosis on older Americans and their families: analysis of the health and retirement study.
      ]. Progress in the area has been hindered by the complex pathogenesis that is not yet fully elucidated. Apart from such biological factors, there remains the larger obstacle that there are no universally accepted standards for the definition, diagnosis, classification, or treatment of HE, mostly as a result of insufficient clinical studies and standardized definitions. Clinical management tends to be dependent on local standards and personal views. This is an unfavorable situation for patients and contrasts with the severity of the condition and the high level of standardization in other complications of cirrhosis. The lack of consistency in the nomenclature and general standards renders comparisons among studies and patient populations difficult, introduces bias, and hinders progress in clinical research for HE. The latest attempts to standardize the nomenclature were published in 2002 and suggestions for the design of HE trials in 2011. Because there is an unmet need for recommendations on the clinical management of HE, the EASL and the AASLD jointly agreed to create these practice guidelines. It is beyond the scope of these guidelines to elaborate on the theories of pathogenesis of HE, as well as the management of encephalopathy resulting from acute liver failure (ALF), which has been published as guidelines recently. Rather, its aim is to present standardized terminology and recommendations to all health care workers who have patients with HE, regardless of their medical discipline, and focus on adult patients with chronic liver disease (CLD), which is, by far, the most frequent scenario.
      As these guidelines on HE were created, the authors found a limited amount of high-quality evidence to extract from the existing literature. There are many reasons for this; the elusive character of HE is among them, as well as the lack of generally accepted and utilized terms for description and categorization of HE. This makes a practice guideline all the more necessary for future improvement of clinical studies and, subsequently, the quality of management of patients with HE. With the existing body of evidence, these guidelines encompass the authors’ best, carefully considered opinions. Although not all readers may necessarily agree with all aspects of the guidelines, their creation and adherence to them is the best way forward, with future adjustments when there is emergence of new evidence.

      Definition of the disease/condition

       Overview

      Advanced liver disease and portosystemic shunting (PSS), far from being an isolated disorder of the liver, have well-known consequences on the body and, notably, on brain functioning. The alterations of brain functioning, which can produce behavioral, cognitive, and motor effects, were termed portosystemic encephalopathy (PSE) [
      • Sherlock S.
      • Summerskill W.H.J.
      • White L.P.
      • Phear E.A.
      Portal-systemic encephalopathy. Neurological complications of liver disease.
      ] and later included in the term HE [
      • Fazekas J.E.
      • Ticktin H.E.
      • Shea J.G.
      Effects of L-arginine on hepatic encephalopathy.
      ].
      Unless the underlying liver disease is successfully treated, HE is associated with poor survival and a high risk of recurrence [
      • Kaplan P.W.
      • Rossetti A.O.
      EEG patterns and imaging correlations in encephalopathy: encephalopathy part II.
      ,
      • Conn H.O.
      Hepatic encephalopathy.
      ]. Even in its mildest form, HE reduces health-related quality of life and is a risk factor for bouts of severe HE [
      • D’Amico G.
      • Morabito A.
      • Pagliaro L.
      • Marubini E.
      Survival and prognostic indicators in compensated and decompensated cirrhosis.
      ,
      • Ding A.
      • Lee A.
      • Callender M.
      • Loughrey M.
      • Quah S.P.
      • Dinsmore W.W.
      Hepatic encephalopathy as an unusual late complication of transjugular intrahepatic portosystemic shunt insertion for non-cirrhotic portal hypertension caused by nodular regenerative hyperplasia in an HIV-positive patient on highly active antiretroviral therapy.
      ,
      • Ito T.
      • Ikeda N.
      • Watanabe A.
      • Sue K.
      • Kakio T.
      • Mimura H.
      • et al.
      Obliteration of portal systemic shunts as therapy for hepatic encephalopathy in patients with non-cirrhotic portal hypertension.
      ].

       Definition of HE

      This definition, in line with previous versions [
      • Ferenci P.
      • Lockwood A.
      • Mullen K.
      • Tarter R.
      • Weissenborn K.
      • Blei A.T.
      Hepatic encephalopathy—definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998.
      ,
      • Cordoba J.
      New assessment of hepatic encephalopathy.
      ], is based on the concept that encephalopathies are “diffuse disturbances of brain function” [
      • Kaplan P.W.
      • Rossetti A.O.
      EEG patterns and imaging correlations in encephalopathy: encephalopathy part II.
      ] and that the adjective “hepatic” implies a causal connection to liver insufficiency and/or perihepatic vascular shunting [
      • Conn H.O.
      Hepatic encephalopathy.
      ].

       Epidemiology

      The incidence and prevalence of HE are related to the severity of the underlying liver insufficiency and PSS [
      • Rikkers L.
      • Jenko P.
      • Rudman D.
      • Freides D.
      Subclinical hepatic encephalopathy: detection, prevalence, and relationship to nitrogen metabolism.
      ,
      • Del Piccolo F.
      • Sacerdoti D.
      • Amodio P.
      • Bombonato G.
      • Bolognesi M.
      • Mapelli D.
      • et al.
      Central nervous system alterations in liver cirrhosis: the role of portal-systemic shunt and portal hypoperfusion.
      ,
      • Bajaj J.S.
      • Schubert C.M.
      • Heuman D.M.
      • Wade J.B.
      • Gibson D.P.
      • Topaz A.
      • et al.
      Persistence of cognitive impairment after resolution of overt hepatic encephalopathy.
      ,
      • Riggio O.
      • Ridola L.
      • Pasquale C.
      • Nardelli S.
      • Pentassuglio I.
      • Moscucci F.
      • et al.
      Evidence of persistent cognitive impairment after resolution of overt hepatic encephalopathy.
      ]. In patients with cirrhosis, fully symptomatic overt HE (OHE) is an event that defines the decompensated phase of the disease, such as VB or ascites [
      • D’Amico G.
      • Morabito A.
      • Pagliaro L.
      • Marubini E.
      Survival and prognostic indicators in compensated and decompensated cirrhosis.
      ]. Overt hepatic encephalopathy is also reported in subjects without cirrhosis with extensive PSS [
      • Ding A.
      • Lee A.
      • Callender M.
      • Loughrey M.
      • Quah S.P.
      • Dinsmore W.W.
      Hepatic encephalopathy as an unusual late complication of transjugular intrahepatic portosystemic shunt insertion for non-cirrhotic portal hypertension caused by nodular regenerative hyperplasia in an HIV-positive patient on highly active antiretroviral therapy.
      ,
      • Ito T.
      • Ikeda N.
      • Watanabe A.
      • Sue K.
      • Kakio T.
      • Mimura H.
      • et al.
      Obliteration of portal systemic shunts as therapy for hepatic encephalopathy in patients with non-cirrhotic portal hypertension.
      ].
      The manifestation of HE may not be an obvious clinical finding and there are multiple tools used for its detection, which influences the variation in the reported incidence and prevalence rates.
      The prevalence of OHE at the time of diagnosis of cirrhosis is 10%–14% in general [
      • Saunders J.B.
      • Walters J.R.F.
      • Davies P.
      • Paton A.
      A 20-year prospective study of cirrhosis.
      ,
      • Romero-Gomez M.
      • Boza F.
      • Garcia-Valdecasas M.S.
      • et al.
      Subclinical hepatic encephalopathy predicts the development of overt hepatic encephalopathy.
      ,
      • Jepsen P.
      • Ott P.
      • Andersen P.K.
      • Sørensen H.T.
      • Vilstrup H.
      The clinical course of alcoholic liver cirrhosis: a Danish population-based cohort study.
      ], 16%–21% in those with decompensated cirrhosis [
      • D’Amico G.
      • Morabito A.
      • Pagliaro L.
      • Marubini E.
      Survival and prognostic indicators in compensated and decompensated cirrhosis.
      ,
      • Coltorti M.
      • Del Vecchio-Blanco C.
      • Caporaso N.
      • Gallo C.
      • Castellano L.
      Liver cirrhosis in Italy. A multicentre study on presenting modalities and the impact on health care resources. National Project on Liver Cirrhosis Group.
      ], and 10%–50% in patients with transjugular intrahepatic portosystemic shunt (TIPS) [
      • Papatheodoridis G.V.
      • Goulis J.
      • Leandro G.
      • Patch D.
      • Burroughs A.K.
      Transjugular intrahepatic portosystemic shunt compared with endoscopic treatment for prevention of variceal rebleeding: a meta-analysis.
      ,
      • Nolte W.
      • Wiltfang J.
      • Schindler C.
      • Münke H.
      • Unterberg K.
      • Zumhasch U.
      • et al.
      Portosystemic hepatic encephalopathy after transjugular intrahepatic portosystemic shunt in patients with cirrhosis: clinical, laboratory, psychometric, and electroencephalographic investigations.
      ]. The cumulated numbers indicate that OHE will occur in 30%–40% of those with cirrhosis at some time during their clinical course and in the survivors in most cases repeatedly [
      • Amodio P.
      • Del Piccolo F.
      • Pettenò E.
      • Mapelli D.
      • Angeli P.
      • Iemmolo R.
      • et al.
      Prevalence and prognostic value of quantified electroencephalogram (EEG) alterations in cirrhotic patients.
      ]. Minimal HE (MHE) or covert HE (CHE) occurs in 20%–80% of patients with cirrhosis [
      • Groeneweg M.
      • Moerland W.
      • Quero J.C.
      • Krabbe P.F.
      • Schalm S.W.
      Screening of subclinical hepatic encephalopathy.
      ,
      • Saxena N.
      • Bhatia M.
      • Joshi Y.K.
      • Garg P.K.
      • Tandon R.K.
      Auditory P300 event-related potentials and number connection test for evaluation of subclinical hepatic encephalopathy in patients with cirrhosis of the liver: a follow-up study.
      ,
      • Schomerus H.
      • Hamster W.
      Quality of life in cirrhotics with minimal hepatic encephalopathy.
      ,
      • Sharma P.
      • Sharma B.C.
      • Puri V.
      • Sarin S.K.
      Critical flicker frequency: diagnostic tool for minimal hepatic encephalopathy.
      ,
      • Bajaj J.S.
      Management options for minimal hepatic encephalopathy.
      ,
      • Romero-Gomez M.
      • Cordoba J.
      • Jover R.
      • del Olmo J.A.
      • Ramirez M.
      • Rey R.
      • et al.
      Value of the critical flicker frequency in patients with minimal hepatic encephalopathy.
      ]. The prevalence of HE in prehepatic noncirrhotic portal hypertension (PH) is not well defined.
      The risk for the first bout of OHE is 5%–25% within 5 years after cirrhosis diagnosis, depending on the presence of risk factors, such as other complications to cirrhosis (MHE or CHE, infections, VB, or ascites) and probably diabetes and hepatitis C [
      • Bustamante J.
      • Rimola A.
      • Ventura P.J.
      • Navasa M.
      • Cirera I.
      • Reggiardo V.
      • et al.
      Prognostic significance of hepatic encephalopathy in patients with cirrhosis.
      ,
      • Hartmann I.J.
      • Groeneweg M.
      • Quero J.C.
      • Beijeman S.J.
      • de Man R.A.
      • Hop W.C.
      • et al.
      The prognostic significance of subclinical hepatic encephalopathy.
      ,
      • Gentilini P.
      • Laffi G.
      • La Villa G.
      • Romanelli R.G.
      • Buzzelli G.
      • Casini-Raggi V.
      • et al.
      Long course and prognostic factors of virus-induced cirrhosis of the liver.
      ,
      • Benvegnoù L.
      • Gios M.
      • Boccato S.
      • Alberti A.
      Natural history of compensated viral cirrhosis: a prospective study on the incidence and hierarchy of major complications.
      ,
      • Watson H.
      • Jepsen P.
      • Wong F.
      • Gines P.
      • Cordoba J.
      • Vilstrup H.
      Satavaptan treatment for ascites in patients with cirrhosis: a meta-analysis of effect on hepatic encephalopathy development.
      ]. Subjects with a previous bout of OHE were found to have a 40% cumulative risk of recurring OHE at 1 year [
      • Sharma B.C.
      • Sharma P.
      • Agrawal A.
      • Sarin S.K.
      Secondary prophylaxis of hepatic encephalopathy: an open-label randomized controlled trial of lactulose vs. placebo.
      ], and subjects with recurrent OHE have a 40% cumulative risk of another recurrence within 6 months, despite lactulose treatment. Even individuals with cirrhosis and only mild cognitive dysfunction or mild electroencephalography (EEG) slowing develop approximately one bout of OHE per 3 years of survival [
      • Prasad S.
      • Dhiman R.K.
      • Duseja A.
      • Chawla Y.K.
      • Sharma A.
      • Agarwal R.
      Lactulose improves cognitive functions and health-related quality of life in patients with cirrhosis who have minimal hepatic encephalopathy.
      ,
      • Amodio P.
      • Pellegrini A.
      • Ubiali E.
      • Mathy I.
      • Piccolo F.D.
      • Orsato R.
      • et al.
      The EEG assessment of low-grade hepatic encephalopathy: comparison of an artificial neural network-expert system (ANNES) based evaluation with visual EEG readings and EEG spectral analysis.
      ].
      After TIPS, the median cumulative 1-year incidence of OHE is 10%–50% [
      • Boyer T.D.
      • Haskal Z.J.
      The role of transjugular intrahepatic portosystemic shunt (TIPS) in the management of portal hypertension: update 2009.
      ,
      • Riggio O.
      • Angeloni S.
      • Salvatori F.M.
      • De S.A.
      • Cerini F.
      • Farcomeni A.
      • et al.
      Incidence, natural history, and risk factors of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt with polytetrafluoroethylene-covered stent grafts.
      ] and is greatly influenced by the patient selection criteria adopted [
      • Bai M.
      • Qi X.
      • Yang Z.
      • Yin Z.
      • Nie Y.
      • Yuan S.
      • et al.
      Predictors of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt in cirrhotic patients: a systematic review.
      ]. Comparable data were obtained by PSS surgery [
      • Spina G.
      • Santambrogio R.
      The role of portosystemic shunting in the management of portal hypertension.
      ].
      It gives an idea of the frequent confrontation of the health care system by patients with HE that they accounted for approximately 110,000 hospitalizations yearly (2005–2009) [
      • Stepanova M.
      • Mishra A.
      • Venkatesan C.
      • Younossi Z.M.
      In-hospital mortality and economic burden associated with hepatic encephalopathy in the United States from 2005 to 2009.
      ] in the United States. Though numbers in the European Union (EU) are not readily available, these predictions are expected to be similar. Furthermore, the burden of CLD and cirrhosis is rapidly increasing [
      • Kim W.R.
      • Brown Jr., R.S.
      • Terrault N.A.
      • El-Serag H.
      Burden of liver disease in the United States: summary of a workshop.
      ,
      • Fleming K.M.
      • Aithal G.P.
      • Solaymani-Dodaran M.
      • Card T.R.
      • West J.
      Incidence and prevalence of cirrhosis in the United Kingdom, 1992–2001: a general population-based study.
      ], and more cases will likely be encountered to further define the epidemiology of HE.

       Clinical presentation

      Hepatic encephalopathy produces a wide spectrum of nonspecific neurological and psychiatric manifestations [
      • Ferenci P.
      • Lockwood A.
      • Mullen K.
      • Tarter R.
      • Weissenborn K.
      • Blei A.T.
      Hepatic encephalopathy—definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998.
      ]. In its lowest expression [
      • Gitlin N.
      • Lewis D.C.
      • Hinkley L.
      The diagnosis and prevalence of sub-clinical hepatic encephalopathy in apparently healthy, ambulant, non-shunted patients with cirrhosis.
      ,
      • Lockwood A.H.
      “What’s in a name?” Improving the care of cirrhotics.
      ], HE alters only psychometric tests oriented toward attention, working memory (WM), psychomotor speed, and visuospatial ability, as well as electrophysiological and other functional brain measures [
      • Amodio P.
      • Montagnese S.
      • Gatta A.
      • Morgan M.Y.
      Characteristics of minimal hepatic encephalopathy.
      ,
      • McCrea M.
      • Cordoba J.
      • Vessey G.
      • Blei A.T.
      • Randolph C.
      Neuropsycho-logical characterization and detection of subclinical hepatic encephalopathy.
      ].
      As HE progresses, personality changes, such as apathy, irritability, and disinhibition, may be reported by the patient’s relatives [
      • Wiltfang J.
      • Nolte W.
      • Weissenborn K.
      • Kornhuber J.
      • Ruther E.
      Psychiatric aspects of portal-systemic encephalopathy.
      ], and obvious alterations in consciousness and motor function occur. Disturbances of the sleep-wake cycle with excessive daytime sleepiness are frequent [
      • Montagnese S.
      • De Pitta C.
      • De Rui M.
      • Corrias M.
      • Turco M.
      • Merkel C.
      • et al.
      Sleep-wake abnormalities in patients with cirrhosis.
      ], whereas complete reversal of the sleep-wake cycle is less consistently observed [
      • Cordoba J.
      • Cabrera J.
      • Lataif L.
      • Penev P.
      • Zee P.
      • Blei A.T.
      High prevalence of sleep disturbance in cirrhosis.
      ,
      • Montagnese S.
      • Middleton B.
      • Skene D.J.
      • Morgan M.Y.
      Night-time sleep disturbance does not correlate with neuropsychiatric impairment in patients with cirrhosis.
      ]. Patients may develop progressive disorientation to time and space, inappropriate behavior, and acute confusional state with agitation or somnolence, stupor, and, finally, coma [
      • Weissenborn K.
      Diagnosis of encephalopathy.
      ]. The recent ISHEN (International Society for Hepatic Encephalopathy and Nitrogen Metabolism) consensus uses the onset of disorientation or asterixis as the onset of OHE [
      • Bajaj J.S.
      • Wade J.B.
      • Sanyal A.J.
      Spectrum of neurocognitive impairment in cirrhosis: implications for the assessment of hepatic encephalopathy.
      ].
      In noncomatose patients with HE, motor system abnormalities, such as hypertonia, hyper-reflexia, and a positive Babinski sign, can be observed. In contrast, deep tendon reflexes may diminish and even disappear in coma [
      • Adams R.D.
      • Foley J.M.
      The neurological disorder associated with liver disease.
      ], although pyramidal signs can still be observed. Rarely, transient focal neurological deficits can occur [
      • Cadranel J.F.
      • Lebiez E.
      • Di Martino V.
      • Bernard B.
      • El Koury S.
      • Tourbah A.
      • et al.
      Focal neurological signs in hepatic encephalopathy in cirrhotic patients: an underestimated entity?.
      ]. Seizures are very rarely reported in HE [
      • Delanty N.
      • French J.A.
      • Labar D.R.
      • Pedley T.A.
      • Rowan A.J.
      Status epilepticus arising de novo in hospitalized patients: an analysis of 41 patients.
      ,
      • Eleftheriadis N.
      • Fourla E.
      • Eleftheriadis D.
      • Karlovasitou A.
      Status epilepticus as a manifestation of hepatic encephalopathy.
      ,
      • Prabhakar S.
      • Bhatia R.
      Management of agitation and convulsions in hepatic encephalopathy.
      ].
      Extrapyramidal dysfunction, such as hypomimia, muscular rigidity, bradykinesia, hypokinesia, monotony and slowness of speech, parkinsonian-like tremor, and dyskinesia with diminished voluntary movements, are common findings; in contrast, the presence of involuntary movements similar to tics or chorea occur rarely [
      • Adams R.D.
      • Foley J.M.
      The neurological disorder associated with liver disease.
      ,
      • Weissenborn K.
      • Bokemeyer M.
      • Krause J.
      • Ennen J.
      • Ahl B.
      Neurological and neuropsychiatric syndromes associated with liver disease.
      ].
      Asterixis or “flapping tremor” is often present in the early to middle stages of HE that precede stupor or coma and is, in actuality, not a tremor, but a negative myoclonus consisting of loss of postural tone. It is easily elicited by actions that require postural tone, such as hyperextension of the wrists with separated fingers or the rhythmic squeezing of the examiner’s fingers. However, asterixis can be observed in other areas, such as the feet, legs, arms, tongue, and eyelids. Asterixis is not pathognomonic of HE because it can be observed in other diseases [
      • Weissenborn K.
      • Bokemeyer M.
      • Krause J.
      • Ennen J.
      • Ahl B.
      Neurological and neuropsychiatric syndromes associated with liver disease.
      ] (e.g., uremia).
      Notably, the mental (either cognitive or behavioral) and motor signs of HE may not be expressed, or do not progress in parallel, in each individual, therefore producing difficulties in staging the severity of HE.
      Hepatic myelopathy (HM) [
      • Read A.E.
      • Sherlock S.
      • Laidlaw J.
      • Walker J.G.
      The neuro-psychiatric syndromes associated with chronic liver disease and an extensive portal-systemic collateral circulation.
      ] is a particular pattern of HE possibly related to marked, long-standing portocaval shunting, characterized by severe motor abnormalities exceeding the mental dysfunction. Cases of paraplegia with progressive spasticity and weakness of lower limbs with hyper-reflexia and relatively mild persistent or recurrent mental alterations have been reported and do not respond to standard therapy, including ammonia lowering, but may reverse with liver transplantation (LT) [
      • Baccarani U.
      • Zola E.
      • Adani G.L.
      • Cavalletti M.
      • Schiff S.
      • Cagnin A.
      • et al.
      Reversal of hepatic myelopathy after liver transplantation: fifteen plus one.
      ].
      Persistent HE may present with prominent extrapyramidal and/or pyramidal signs, partially overlapping with HM, in which postmortem brain examination reveals brain atrophy [
      • Victor M.
      • Adams R.D.
      • Cole M.
      The acquired (non Wilsonian) type of chronic hepatocerebral degeneration.
      ]. This condition was previously called acquired hepatolenticular degeneration, a term currently considered obsolete. However, this cirrhosis-associated parkinsonism is unresponsive to ammonia-lowering therapy and may be more common than originally thought in patients with advanced liver disease, presenting in approximately 4% of cases [
      • Tryc A.B.
      • Goldbecker A.
      • Berding G.
      • Rümke S.
      • Afshar K.
      • Shahrezaei G.H.
      • et al.
      Cirrhosis-related Parkinsonism: prevalence, mechanisms and response to treatments.
      ].
      Apart from these less-usual manifestations of HE, it is widely accepted in clinical practice that all forms of HE and their manifestations are completely reversible, and this assumption still is a well-founded operational basis for treatment strategies. However, research on liver-transplanted HE patients and on patients after resolution of repeated bouts of OHE casts doubt on the full reversibility. Some mental deficits, apart from those ascribable to other transplantation-related causes, may persist and are mentioned later under transplantation [
      • Garcia-Martinez R.
      • Rovira A.
      • Alonso J.
      • Jacas C.
      • Simón-Talero M.
      • Chavarria L.
      • et al.
      Hepatic encephalopathy is associated with posttransplant cognitive function and brain volume.
      ]. Likewise, episodes of OHE may be associated with persistent cumulative deficits in WM and learning [
      • Bajaj J.S.
      • Schubert C.M.
      • Heuman D.M.
      • Wade J.B.
      • Gibson D.P.
      • Topaz A.
      • et al.
      Persistence of cognitive impairment after resolution of overt hepatic encephalopathy.
      ].

       Classification

      Hepatic encephalopathy should be classified according to all of the following four factors [
      • Ferenci P.
      • Lockwood A.
      • Mullen K.
      • Tarter R.
      • Weissenborn K.
      • Blei A.T.
      Hepatic encephalopathy—definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998.
      ].
      • (1)
        According to the underlying disease, HE is subdivided into
        • Type A resulting from ALF
        • Type B resulting predominantly from portosystemic bypass or shunting
        • Type C resulting from cirrhosis
      • The clinical manifestations of types B and C are similar, whereas type A has distinct features and, notably, may be associated with increased intracranial pressure and a risk of cerebral herniation. The management of HE type A is described in recent guidelines on ALF [
        • Lee W.M.
        • Stravitz R.T.
        • Larson A.M.
        Introduction to the revised American Association for the Study of Liver Diseases Position Paper on acute liver failure 2011.
        ,

        American Association for the Study of Liver Diseases Position Paper on acute liver failure 2011. Full text. Available at: www.aasld.org/practice-guidelines/Documents/AcuteLiverFailureUpdate2011.pdf.

        ] and is not included in this document.
      • (2)
        According to the severity of manifestations. The continuum that is HE has been arbitrarily subdivided. For clinical and research purposes, a scheme of such grading is provided (Table 2). Operative classifications that refer to defined functional impairments aim at increasing intra- and inter-rater reliability and should be used whenever possible.
        Table 2WHC and clinical description.
        All conditions are required to be related to liver insufficiency and/or PSS.
      • (3)
        According to its time course, HE is subdivided into
        • Episodic HE
        • Recurrent HE denotes bouts of HE that occur with a time interval of 6 months or less.
        • Persistent HE denotes a pattern of behavioral alterations that are always present and interspersed with relapses of overt HE.
      • (4)
        According to the existence of precipitating factors, HE is subdivided into
        • Nonprecipitated or
        • Precipitated, and the precipitating factors should be specified. Precipitating factors can be identified in nearly all bouts of episodic HE type C and should be actively sought and treated when found (Table 3).
          Table 3Precipitating factors for OHE by decreasing frequency.
          Modified from Strauss E, da Costa MF The importance of bacterial infections as precipitating factors of chronic hepatic encephalopathy in cirrhosis. Hepato-gastroenterology 1998;45:900–904.
          More recent unpublished case series confirm the dominant role of infections.
      A fifth classification, according to whether or not the patient has acute-on-chronic liver failure (ACLF), has recently been suggested [
      • Cordoba J.
      • Ventura-Cots M.
      • Simón-Talero M.
      • Amorós A.
      • Pavesi M.
      • Vilstrup H.
      • et al.
      CANONIC Study Investigators of the EASL-CLIF Consortium. Characteristics, risk factors, and mortality of cirrhotic patients hospitalized for hepatic encephalopathy with and without acute-on-chronic liver failure (ACLF).
      ]. Although the management, mechanism, and prognostic impact differ, this classification is still a research area.

       Differential diagnoses

      The diagnosis requires the detection of signs suggestive of HE in a patient with severe liver insufficiency and/or PSS who does not have obvious alternative causes of brain dysfunction. The recognition of precipitating factors for HE (e.g., infection, bleeding, and constipation) supports the diagnosis of HE. The differential diagnosis should consider common disorders altering the level of consciousness (Table 4).
      Table 4Differential diagnosis of HE.
      Hyponatremia and sepsis can both produce encephalopathy per se and precipitate HE by interactions with the pathophysiological mechanisms. In end-stage liver disease, uremic encephalopathy and HE may overlap.

       Recommendations

      Every case and bout of HE should be described and classified according to all four factors, and this should be repeated at relevant intervals according to the clinical situation. The recommendations are summarized in Table 5.
      Table 5HE description and clinical example.
      The HE patient should be characterized by one component from each of the four columns. Example of a recommended description of a patient with HE: “The patient has HE, Type C, Grade 3, Recurrent, Precipitated (by urinary tract infection).” The description may be supplemented with operative classifications (e.g., the Glasgow Coma Score or psychometric performance).

      Diagnosis and testing

       Clinical evaluation

      Judging and measuring the severity of HE is approached as a continuum [
      • Bajaj J.S.
      • Wade J.B.
      • Sanyal A.J.
      Spectrum of neurocognitive impairment in cirrhosis: implications for the assessment of hepatic encephalopathy.
      ]. The testing strategies in place range from simple clinical scales to sophisticated psychometric and neurophysiological tools; however, none of the current tests are valid for the entire spectrum [
      • Cordoba J.
      New assessment of hepatic encephalopathy.
      ,
      • Bajaj J.S.
      • Cordoba J.
      • Mullen K.D.
      • Amodio P.
      • Shawcross D.L.
      • Butterrworth R.F.
      • et al.
      Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
      ]. The appropriate testing and diagnostic options differ according to the acuity of the presentation and the degree of impairment [
      • Montagnese S.
      • Amodio P.
      • Morgan M.Y.
      Methods for diagnosing hepatic encephalopathy in patients with cirrhosis: a multidimensional approach.
      ].

       Diagnosis and testing for OHE

      The diagnosis of OHE is based on a clinical examination and a clinical decision. Clinical scales are used to analyze its severity. Specific quantitative tests are only needed in study settings. The gold standard is the West Haven criteria (WHC; Table 2, including clinical description). However, they are subjective tools with limited interobserver reliability, especially for grade I HE, because slight hypokinesia, psychomotor slowing, and a lack of attention can easily be overlooked in clinical examination. In contrast, the detection of disorientation and asterixis has good inter-rater reliability and thus are chosen as marker symptoms of OHE [
      • Montagnese S.
      • Amodio P.
      • Morgan M.Y.
      Methods for diagnosing hepatic encephalopathy in patients with cirrhosis: a multidimensional approach.
      ]. Orientation or mixed scales have been used to distinguish the severity of HE [
      • Prakash R.
      • Mullen K.D.
      Mechanisms, diagnosis and management of hepatic encephalopathy.
      ,
      • Hassanein T.I.
      • Hilsabeck R.C.
      • Perry W.
      Introduction to the Hepatic Encephalopathy Scoring Algorithm (HESA).
      ]. In patients with significantly altered consciousness, the Glasgow Coma Scale (GCS; Table 6) is widely employed and supplies an operative, robust description.
      Table 6GCS
      • Teasdale G.
      • Jennett B.
      Assessment of coma and impaired consciousness: a practical scale.
      .
      The scale comprises three tests: eyes, verbal, and motor responses. The three values separately as well as their sum are considered. The lowest possible GCS (the sum) is 3 (deep coma or death), whereas the highest is 15 (fully awake person). Abbreviation: n.a., not applicable.
      Diagnosing cognitive dysfunction is not difficult. It can be established from clinical observation as well as neuropsychological or neurophysiological tests. The difficulty is to assign them to HE. For this reason, OHE still remains a diagnosis of exclusion in this patient population that is often susceptible to mental status abnormalities resulting from medications, alcohol abuse, drug use, effects of hyponatremia, and psychiatric disease (Table 4). Therefore, as clinically indicated, exclusion of other etiologies by laboratory and radio logical assessment for a patient with altered mental status in HE is warranted.

       Testing for MHE and CHE

      Minimal hepatic encephalopathy and CHE is defined as the presence of test-dependent or clinical signs of brain dysfunction in patients with CLD who are not disoriented or display asterixis. The term “minimal” conveys that there is no clinical sign, cognitive or other, of HE. The term “covert” includes minimal and grade 1 HE. Testing strategies can be divided into two major types: psychometric and neurophysiological [
      • Guerit J.M.
      • Amantini A.
      • Fischer C.
      • Kaplan P.W.
      • Mecarelli O.
      • Schnitzler A.
      • et al.
      Neurophysiological investigations of hepatic encephalopathy: ISHEN practice guidelines.
      ,
      • Randolph C.
      • Hilsabeck R.
      • Kato A.
      • Kharbanda P.
      • Li Y.Y.
      • Mapelli D.
      • et al.
      Neuropsychological assessment of hepatic encephalopathy: ISHEN practice guidelines.
      ]. Because the condition affects several components of cognitive functioning, which may not be impaired to the same degree, the ISHEN suggests the use of at least two tests, depending on the local population norms and availability, and preferably with one of the tests being more widely accepted so as to serve as a comparator.
      Testing for MHE and CHE is important because it can prognosticate OHE development, indicate poor quality of life and reduced socioeconomic potential, and help counsel patients and caregivers about the disease. The occurrence of MHE and CHE in patients with CLD seems to be as high as 50% [
      • Lauridsen M.M.
      • Jepsen P.
      • Vilstrup H.
      Critical flicker frequency and continuous reaction times for the diagnosis of minimal hepatic encephalopathy: a comparative study of 154 patients with liver disease.
      ], so, ideally, every patient at risk should be tested. However, this strategy may be costly [
      • Bajaj J.S.
      • Pinkerton S.D.
      • Sanyal A.J.
      • Heuman D.M.
      Diagnosis and treatment of minimal hepatic encephalopathy to prevent motor vehicle accidents: a cost-effectiveness analysis.
      ], and the consequences of the screening procedure are not always clear and treatment is not always recommended. An operational approach may be to test patients who have problems with their quality of life or in whom there are complaints from the patients and their relatives [
      • Ortiz M.
      • Jacas C.
      • Cordoba J.
      Minimal hepatic encephalopathy: diagnosis, clinical significance and recommendations.
      ]. Tests positive for MHE or CHE before stopping HE drug therapy will identify patients at risk for recurrent HE [
      • Sharma B.C.
      • Sharma P.
      • Agrawal A.
      • Sarin S.K.
      Secondary prophylaxis of hepatic encephalopathy: an open-label randomized controlled trial of lactulose vs. placebo.
      ,
      • Bajaj J.S.
      • Gillevet P.M.
      • Patel N.R.
      • Ahluwalia V.
      • Ridlon J.M.
      • Kettenmann B.
      • et al.
      A longitudinal systems biology analysis of lactulose withdrawal in hepatic encephalopathy.
      ]. Furthermore, none of the available tests are specific for the condition [
      • Weissenborn K.
      • Ennen J.C.
      • Schomerus H.
      • Ruckert N.
      • Hecker H.
      Neuropsychological characterization of hepatic encephalopathy.
      ], and it is important to test only patients who do not have confounding factors, such as neuropsychiatric disorders, psychoactive medication, or current alcohol use.
      Testing should be done by a trained examiner adhering to scripts that accompany the testing tools. If the test result is normal (i.e., negative for MHE or CHE), repeat testing in 6 months has been recommended [
      • Prakash R.K.
      • Brown T.A.
      • Mullen K.D.
      Minimal hepatic encephalopathy and driving: is the genie out of the bottle?.
      ]. A diagnosis of MHE or CHE does not automatically mean that the affected subject is a dangerous driver [
      • Bajaj J.S.
      • Stein A.C.
      • Dubinsky R.M.
      What is driving the legal interest in hepatic encephalopathy?.
      ]. Medical providers are not trained to formally evaluate fitness to drive and are also not legal representatives. Therefore, providers should act in the best interests of both the patient and society while following the applicable local laws [
      • Bajaj J.S.
      • Stein A.C.
      • Dubinsky R.M.
      What is driving the legal interest in hepatic encephalopathy?.
      ]. However, doctors cannot evade the responsibility of counseling patients with diagnosed HE on the possible dangerous consequences of their driving, and, often, the safest advice is to stop driving until the responsible driving authorities have formally cleared the patient for safe driving. In difficult cases, the doctor should consult with the authorities that have the expertise to test driving ability and the authority to revoke the license.
      A listing of the most established testing strategies is given below. The test recommendation varies depending on the logistics, availability of tests, local norms, and cost [
      • Bajaj J.S.
      • Wade J.B.
      • Sanyal A.J.
      Spectrum of neurocognitive impairment in cirrhosis: implications for the assessment of hepatic encephalopathy.
      ,
      • Bajaj J.S.
      • Cordoba J.
      • Mullen K.D.
      • Amodio P.
      • Shawcross D.L.
      • Butterrworth R.F.
      • et al.
      Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
      ,
      • Randolph C.
      • Hilsabeck R.
      • Kato A.
      • Kharbanda P.
      • Li Y.Y.
      • Mapelli D.
      • et al.
      Neuropsychological assessment of hepatic encephalopathy: ISHEN practice guidelines.
      ].
      • (1)
        Portosystemic encephalopathy (PSE) syndrome test. This test battery consists of five paper-pencil tests that evaluate cognitive and psychomotor processing speed and visuomotor coordination. The tests are relatively easy to administer and have good external validity [
        • Weissenborn K.
        • Ennen J.C.
        • Schomerus H.
        • Ruckert N.
        • Hecker H.
        Neuropsychological characterization of hepatic encephalopathy.
        ]. The test is often referred to as the Psychometric Hepatic Encephalopathy Score (PHES), with the latter being the sum score from all subtests of the battery. It can be obtained from Hannover Medical School (Hannover, Germany), which holds the copyright ([email protected]). The test was developed in Germany and has been translated for use in many other countries. For illiterate patients, the figure connection test has been used as a subtest instead of the number connection test [
        • Dhiman R.K.
        • Saraswat V.A.
        • Verma M.
        • Naik S.R.
        Figure connection test: a universal test for assessment of mental state.
        ].
      • (2)
        The Critical Flicker Frequency (CFF) test is a psychophysiological tool defined as the frequency at which a fused light (presented from 60 Hz downward) appears to be flickering to the observer. Studies have shown its reduction with worsening cognition and improvement after therapy. The CFF test requires several trials, intact binocular vision, absence of red-green blindness, and specialized equipment [
        • Kircheis G.
        • Wettstein M.
        • Timmermann L.
        • Schnitzler A.
        • Haussinger D.
        Critical flicker frequency for quantification of low-grade hepatic encephalopathy.
        ,
        • Romero-Gomez M.
        • Cordoba J.
        • Jover R.
        • del Olmo J.A.
        • Ramirez M.
        • Rey R.
        • et al.
        Value of the critical flicker frequency in patients with minimal hepatic encephalopathy.
        ].
      • (3)
        The Continuous Reaction Time (CRT) test. The CRT test relies on repeated registration of the motor reaction time (pressing a button) to auditory stimuli (through headphones). The most important test result is the CRT index, which measures the stability of the reaction times. The test result can differentiate between organic and metabolic, brain impairment and is not influenced by the patient’s age or gender, and there is no learning or tiring effect. Simple software and hardware are required [
        • Lauridsen M.M.
        • Thiele M.
        • Kimer N.
        • Vilstrup H.
        The continuous reaction times method for diagnosing, grading, and monitoring minimal/covert hepatic encephalopathy.
        ].
      • (4)
        The Inhibitory Control Test (ICT) is a computerized test of response inhibition and working memory [
        • Bajaj J.S.
        • Hafeezullah M.
        • Franco J.
        • Varma R.R.
        • Hoffmann R.G.
        • Knox J.F.
        • et al.
        Inhibitory control test for the diagnosis of minimal hepatic encephalopathy.
        ] and is freely downloadable at www.hecme.tv. The ICT test has been judged to have good validity, but requires highly functional patients. The norms for the test have to be elaborated beyond the few centers that have used it.
      • (5)
        The Stroop test evaluates psychomotor speed and cognitive flexibility by the interference between recognition reaction time to a colored field and a written color name. Recently, mobile application software (“apps” for a smartphone or tablet computer) based on the test has been shown to identify cognitive dysfunction in cirrhosis compared to paper-pencil tests [
        • Bajaj J.S.
        • Thacker L.R.
        • Heumann D.M.
        • Fuchs M.
        • Sterling R.K.
        • Sanyal A.J.
        • et al.
        The Stroop smartphone application is a short and valid method to screen for minimal hepatic encephalopathy.
        ]. Further studies are under way to evaluate its potential for screening for MHE and CHE.
      • (6)
        The SCAN Test is a computerized test that measures speed and accuracy to perform a digit recognition memory task of increasing complexity. The SCAN Test has been shown to be of prognostic value [
        • Amodio P.
        • Del Piccolo F.
        • Marchetti P.
        • Angeli P.
        • Iemmolo R.
        • Caregaro L.
        • et al.
        Clinical features and survival of cirrhotic patients with sub-clinical cognitive alterations detected by the number connection test and computerized psychometric tests.
        ].
      • (7)
        Electroencephalography examination can detect changes in cortical cerebral activity across the spectrum of HE without patient cooperation or risk of a learning effect [
        • Guerit J.M.
        • Amantini A.
        • Fischer C.
        • Kaplan P.W.
        • Mecarelli O.
        • Schnitzler A.
        • et al.
        Neurophysiological investigations of hepatic encephalopathy: ISHEN practice guidelines.
        ]. However, it is nonspecific and may be influenced by accompanying metabolic disturbances, such as hyponatremia as well as drugs. Possibly, the reliability of EEG analysis can increase with quantitative analysis. This specifically should include the background frequency with mean dominant frequency or spectral band analysis [
        • Victor M.
        • Adams R.D.
        • Cole M.
        The acquired (non Wilsonian) type of chronic hepatocerebral degeneration.
        ]. Also, in most situations, EEG requires an institutional setup and neurological expertise in evaluation, and the cost varies among hospitals.
      Although the above-described tests have been used to test for MHE and CHE, there is, most often, a poor correlation between them because HE is a multi dimensional dysfunction [
      • Montagnese S.
      • Biancardi A.
      • Schiff S.
      • Carraro P.
      • Carla V.
      • Mannaioni G.
      • et al.
      Different biochemical correlates for different neuropsychiatric abnormalities in patients with cirrhosis.
      ]. Learning effect is often observed with psychometric tests and it is unclear whether current HE therapy plays a role in the test performance. Therefore, interpretation of these tests and consideration of the results for further management need an understanding of the patient’s history, current therapy, and effect on the patient’s daily activities, if signs of HE are found. For multicenter studies, the diagnosis of MHE or CHE by consensus should utilize at least two of the current validated testing strategies: paper-pencil (PHES) and one of the following: computerized (CRT, ICT, SCAN, or Stroop) or neurophysiological (CFF or EEG) [
      • Bajaj J.S.
      • Cordoba J.
      • Mullen K.D.
      • Amodio P.
      • Shawcross D.L.
      • Butterrworth R.F.
      • et al.
      Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
      ]. In the clinical routine or single-center studies, investigators may use tests for assessing the severity of HE with which they are familiar, provided that normative reference data are available and the tests have been validated for use in this patient population [
      • Bajaj J.S.
      • Cordoba J.
      • Mullen K.D.
      • Amodio P.
      • Shawcross D.L.
      • Butterrworth R.F.
      • et al.
      Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
      ].

       Laboratory testing

      High blood-ammonia levels alone do not add any diagnostic, staging, or prognostic value in HE patients with CLD [
      • Lockwood A.H.
      Blood ammonia levels and hepatic encephalopathy.
      ]. However, in case an ammonia level is checked in a patient with OHE and it is normal, the diagnosis of HE is in question. For ammonia-lowering drugs, repeated measurements of ammonia may be helpful to test the efficacy. There may be logistic challenges to accurately measure blood ammonia, which should be taken into consideration. Ammonia is reported either in venous, arterial blood, or plasma ammonia, so the relevant normal should be used. Multiple methods are available, but measurements should only be employed when laboratory standards allow for reliable analyses.

       Brain scans

      Computed tomography (CT) or magnetic resonance (MR) or other image modality scans do not contribute diagnostic or grading information. However, the risk of intracerebral hemorrhage is at least 5-fold increased in this patient group [
      • Grønbaek H.
      • Johnsen S.P.
      • Jepsen P.
      • Gislum M.
      • Vilstrup H.
      • Tage-Jensen U.
      • et al.
      Liver cirrhosis, other liver diseases, and risk of hospitalisation for intracerebral haemorrhage: a Danish population-based case-control study.
      ], and the symptoms may be indistinguishable, so a brain scan is usually part of the diagnostic workup of first-time HE and on clinical suspicion of other pathology.

       Recommendations

      Treatment

       General principles

      At this time, only OHE is routinely treated [
      • Ferenci P.
      • Lockwood A.
      • Mullen K.
      • Tarter R.
      • Weissenborn K.
      • Blei A.T.
      Hepatic encephalopathy—definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998.
      ]. Minimal hepatic encephalopathy and CHE, as its title implies, is not obvious on routine clinical examination and is predominantly diagnosed by techniques outlined in the previous section. Despite its subtle nature, MHE and CHE can have a significant effect on a patient’s daily living. Special circumstances can prevail where there may be an indication to treat such a patient (e.g., impairment in driving skills, work performance, quality of life, or cognitive complaints). Liver transplantation is mentioned under the treatment recommendations.

       Recommendations

       Comments on management strategy

      Patients with higher grades of HE who are at risk or unable to protect their airway need more intensive monitoring and are ideally managed in an intensive care setting. Alternative causes of encephalopathy are not infrequent in patients with advanced cirrhosis. Technically, if other causes of encephalopathy are present, then the episode of encephalopathy may not be termed HE. In the clinical setting, what transpires is treatment of both HE and non-HE.
      Controlling precipitating factors in the management of OHE is of paramount importance, because nearly 90% of patients can be treated with just correction of the precipitating factor [
      • Strauss E.
      • Tramote R.
      • Silva E.P.
      • Caly W.R.
      • Honain N.Z.
      • Maffei R.A.
      • et al.
      Double-blind randomized clinical trial comparing neomycin and placebo in the treatment of exogenous hepatic encephalopathy.
      ]. Careful attention to this issue is still the cornerstone of HE management.

       Therapy for episodes of OHE

      In addition to the other elements of the four-pronged approach to treatment of HE, specific drug treatment is part of the management. Most drugs have not been tested by rigorous randomized, controlled studies and are utilized based on circumstantial observations. These agents include nonabsorbable disaccharides, such as lactulose, and antibiotics, such as rifaximin. Other therapies, such as oral branched-chain amino acids (BCAAs), intravenous (IV) L-ornithine L-aspartate (LOLA), probiotics, and other antibiotics, have also been used. In the hospital, a nasogastric tube can be used to administer oral therapies in patients who are unable to swallow or have an aspiration risk.

       Nonabsorbable disaccharides

      Lactulose is generally used as initial treatment for OHE. A large meta-analysis of trial data did not completely support lactulose as a therapeutic agent for treatment of OHE, but for technical reasons, it did not include the largest trials, and these agents continue to be used widely [
      • Als-Nielsen B.
      • Gluud L.L.
      • Gluud C.
      Non-absorbable disaccharides for hepatic encephalopathy: systematic review of randomised trials.
      ]. Lack of effect of lactulose should prompt a clinical search for unrecognized precipitating factors and competing causes for the brain impairment. Though it is assumed that the prebiotic effects (the drug being a nondigestible substance that promotes the growth of beneficial microorganisms in the intestines) and acidifying nature of lactulose have an additional benefit beyond the laxative effect, culture-independent studies have not borne those out [
      • Bajaj J.S.
      • Gillevet P.M.
      • Patel N.R.
      • Ahluwalia V.
      • Ridlon J.M.
      • Kettenmann B.
      • et al.
      A longitudinal systems biology analysis of lactulose withdrawal in hepatic encephalopathy.
      ,
      • Riggio O.
      • Varriale M.
      • Testore G.P.
      • Di Rosa R.
      • Di Rosa E.
      • Merli M.
      • et al.
      Effect of lactitol and lactulose administration on the fecal flora in cirrhotic patients.
      ]. In addition, most recent trials on lactulose have been open label in nature. Cost considerations alone add to the argument in support of lactulose [
      • Huang E.
      • Esrailian E.
      • Spiegel B.M.
      The cost-effectiveness and budget impact of competing therapies in hepatic encephalopathy—a decision analysis.
      ]. In some centers, lactitol is preferred to lactulose, based on small meta-analyses of even smaller trials [
      • Camma C.
      • Fiorello F.
      • Tine F.
      • Marchesini G.
      • Fabbri A.
      • Pagliaro L.
      Lactitol in treatment of chronic hepatic encephalopathy. A meta-analysis.
      ,
      • Morgan M.Y.
      • Hawley K.E.
      • Stambuk D.
      Lactitol vs. lactulose in the treatment of chronic hepatic encephalopathy. A double-blind, randomised, cross-over study.
      ].
      In populations with a high prevalence of lactose intolerance, the use of lactose has been suggested [
      • Uribe M.
      • Berthier J.M.
      • Lewis H.
      • Mata J.M.
      • Sierra J.G.
      • García-Ramos G.
      • et al.
      Lactose enemas plus placebo tablets vs. neomycin tablets plus starch enemas in acute portal systemic encephalopathy. A double-blind randomized controlled study.
      ]. However, the only trial to show that stool-acidifying enemas (lactose and lactulose) were superior to tap-water enemas was underpowered [
      • Uribe M.
      • Campollo O.
      • Vargas F.
      • Ravelli G.P.
      • Mundo F.
      • Zapata L.
      • et al.
      Acidifying enemas (lactitol and lactose) vs. nonacidifying enemas (tap water) to treat acute portal-systemic encephalopathy: a double-blind, randomized clinical trial.
      ]. The use of poly ethylene glycol preparation [
      • Rahimi R.S.
      • Singal A.G.
      • Cuthbert J.A.
      • Rockey D.G.
      A randomized trial of polyethylene glycol 3350-electrolyte solution (PEG) and lactulose for patients hospitalized with acute hepatic encephalopathy.
      ] needs further validation.
      The dosing of lactulose should be initiated [
      • Conn H.O.
      • Lieberthal M.M.
      The hepatic coma syndromes and lactulose.
      ] when the three first elements of the four-pronged approach are completed, with 25 mL of lactulose syrup every 12 h until at least two soft or loose bowel movements per day are produced. Subsequently, the dosing is titrated to maintain two to three bowel movements per day. This dose reduction should be implemented. It is a misconception that lack of effect of smaller amounts of lactulose is remedied by much larger doses. There is a danger for overuse of lactulose leading to complications, such as aspiration, dehydration, hypernatremia, and severe perianal skin irritation, and overuse can even precipitate HE [
      • Bajaj J.S.
      • Sanyal A.J.
      • Bell D.
      • Gilles H.
      • Heuman D.M.
      Predictors of the recurrence of hepatic encephalopathy in lactulose-treated patients.
      ].

       Rifaximin

      Rifaximin has been used for the therapy of HE in a number of trials [
      • Patidar K.R.
      • Bajaj J.S.
      Antibiotics for the treatment of hepatic encephalopathy.
      ] comparing it with placebo, other antibiotics, nonabsorbable disaccharides, and in dose-ranging studies. These trials showed effect of rifaximin that was equivalent or superior to the compared agents with good tolerability. Long-term cyclical therapy over 3–6 months with rifaximin for patients with OHE has also been studied in three trials (two compared to nonabsorbable disaccharides and one against neomycin) showing equivalence in cognitive improvement and ammonia lowering. A multinational study [
      • Bass N.M.
      • Mullen K.D.
      • Sanyal A.
      • Poordad F.
      • Neff G.
      • Leevy C.B.
      • et al.
      Rifaximin treatment in hepatic encephalopathy.
      ] with patients having two earlier OHE bouts to maintain remission showed the superiority of rifaximin vs. placebo (in the background of 91% lactulose use). No solid data support the use of rifaximin alone.

       Other therapies

      Many drugs have been used for treatment of HE, but data to support their use are limited, preliminary, or lacking. However, most of these drugs can safely be used despite their limited proven efficacy.

       BCAAs

      An updated meta-analysis of eight randomized, controlled trials (RCTs) indicated that oral BCAA-enriched formulations improve the manifestations of episodic HE whether OHE or MHE [
      • Gluud L.L.
      • Dam G.
      • Borre M.
      • Les I.
      • Cordoba J.
      • Marchesini G.
      • et al.
      Lactulose, rifaximin or branched chain amino acids for hepatic encephalopathy: what is the evidence?.
      ,
      • Gluud L.L.
      • Dam G.
      • Borre M.
      • Les I.
      • Cordoba J.
      • Marchesini G.
      • et al.
      Oral branched chain amino acids have a beneficial effect on manifestations of hepatic encephalopathy in a systematic review with meta-analyses of randomized controlled trials.
      ]. There is no effect of IV BCAA on the episodic bout of HE [
      • Naylor C.D.
      • O’Rourke K.
      • Detsky A.S.
      • Baker J.P.
      Parenteral nutrition with branched-chain amino acids in hepatic encephalopathy. A meta-analysis.
      ].

       Metabolic ammonia scavengers

      These agents, through their metabolism, act as urea surrogates excreted in urine. Such drugs have been used for treatment of inborn errors of the urea cycle for many years. Different forms are available and currently present as promising investigational agents. Ornithine phenylacetate has been studied for HE, but further clinical reports are awaited [
      • Ventura-Cots M.
      • Arranz J.A.
      • Simόn-Talero M.
      • Torrens M.
      • Blanco A.
      • Riudor E.
      • et al.
      Safety of ornithine phenylacetate in cirrhotic decompensated patients: an open-label, dose-escalating, single-cohort study.
      ]. Glyceryl phenylbutyrate (GPB) was tested in a recent RCT [
      • Rockey D.C.
      • Vierling J.M.
      • Mantry P.
      • Ghabril M.
      • Brown Jr., R.S.
      • Alexeeva O.
      • et al.
      HALT-HE Study Group. Randomized, double-blind, controlled study of glycerol phenylbutyrate in hepatic encephalopathy.
      ] on patients who had experienced two or more episodes of HE in the last 6 months and who were maintained on standard therapy (lactulose ± rifaximin). The GPB arm experienced fewer episodes of HE and hospitalizations as well as longer time to first event. More clinical studies on the same principle are under way and, if confirmed, may lead to clinical recommendations.

       L-ornithine L-aspartate (LOLA)

      An RCT on patients with persistent HE demonstrated improvement by IV LOLA in psychometric testing and postprandial venous ammonia levels [
      • Kircheis G.
      • Nilius R.
      • Held C.
      • Berndt H.
      • Buchner M.
      • Gortelmeyer R.
      • et al.
      Therapeutic efficacy of L-ornithine-L-aspartate infusions in patients with cirrhosis and hepatic encephalopathy: results of a placebo-controlled, double-blind study.
      ]. Oral supplementation with LOLA is ineffective.

       Probiotics

      A recent, open-label study of either lactulose, probiotics, or no therapy in patients with cirrhosis who recovered from HE found fewer episodes of HE in the lactulose or probiotic arms, compared to placebo, but were not different between either interventions. There was no difference in rates of readmission in any of the arms of the study [
      • Agrawal A.
      • Sharma B.C.
      • Sharma P.
      • Sarin S.K.
      Secondary prophylaxis of hepatic encephalopathy in cirrhosis: an open-label, randomized controlled trial of lactulose, probiotics, and no therapy.
      ].

       Glutaminase inhibitors

      Portosystemic shunting up-regulates the intestinal glutaminase gene so that intestinal glutaminase inhibitors may be useful by reducing the amounts of ammonia produced by the gut.

       Neomycin

      This antibiotic still has its advocates and was widely used in the past for HE treatment; it is a known glutaminase inhibitor [
      • Hawkins R.A.
      • Jessy J.
      • Mans A.M.
      • Chedid A.
      • DeJoseph M.R.
      Neomycin reduces the intestinal production of ammonia from glutamine.
      ].

       Metronidazole

      As short-term therapy [
      • Morgan M.H.
      • Read A.E.
      • Speller D.C.
      Treatment of hepatic encephalopathy with metronidazole.
      ], metronidazole also has advocates for its use. However, long-term ototoxicity, nephrotoxicity, and neurotoxicity make these agents unattractive for continuous long-term use.

       Flumazenil

      This drug is not frequently used. It transiently improves mental status in OHE without improvement on recovery or survival. The effect may be of importance in marginal situations to avoid assisted ventilation. Likewise, the effect may be helpful in difficult differential diagnostic situations by confirming reversibility (e.g., when standard therapy unexpectedly fails or when benzodiazepine toxicity is suspected).

       Laxatives

      Simple laxatives alone do not have the prebiotic properties of disaccharides, and no publications have been forthcoming on this issue.

       Albumin

      A recent RCT on OHE patients on rifaximin given daily IV albumin or saline showed no effect on resolution of HE, but was related to better postdischarge survival [
      • Simón-Talero M.
      • García-Martínez R.
      • Torrens M.
      • Augustin S.
      • Gómez S.
      • Pereira G.
      • et al.
      Effects of intravenous albumin in patients with cirrhosis and episodic hepatic encephalopathy: a randomized double-blind study.
      ].

       Recommendations

       Prevention of overt hepatic encephalopathy

       After an episode of OHE

      There are no randomized, placebo-controlled trials of lactulose for maintenance of remission from OHE. However, it is still widely recommended and practiced. A single-center, open-label RCT of lactulose demonstrated less recurrence of HE in patients with cirrhosis [
      • Sharma B.C.
      • Sharma P.
      • Agrawal A.
      • Sarin S.K.
      Secondary prophylaxis of hepatic encephalopathy: an open-label randomized controlled trial of lactulose vs. placebo.
      ]. A recent RCT supports lactulose as prevention of HE subsequent to upper gastrointestinal (GI) bleeding [
      • Sharma P.
      • Agrawal A.
      • Sharma B.C.
      • Sarin S.K.
      Prophylaxis of hepatic encephalopathy in acute variceal bleed: a randomized controlled trial of lactulose vs. no lactulose.
      ].
      Rifaximin added to lactulose is the best-documented agent to maintain remission in patients who have already experienced one or more bouts of OHE while on lactulose treatment after their initial episode of OHE [
      • Bass N.M.
      • Mullen K.D.
      • Sanyal A.
      • Poordad F.
      • Neff G.
      • Leevy C.B.
      • et al.
      Rifaximin treatment in hepatic encephalopathy.
      ].

       Hepatic encephalopathy after TIPS

      Once TIPS was popularized to treat complications of PH, its tendency to cause the appearance of HE, or less commonly, intractable persistent HE, was noted. Faced with severe HE as a complication of a TIPS procedure, physicians had a major dilemma. Initially, it was routine to use standard HE treatment to prevent post-TIPS HE. However, one study illustrated that neither rifaximin nor lactulose prevented post-TIPS HE any better than placebo [
      • Riggio O.
      • Masini A.
      • Efrati C.
      • Nicolao F.
      • Angeloni S.
      • Salvatori F.M.
      • et al.
      Pharmacological prophylaxis of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt: a randomized controlled study.
      ]. Careful case selection has reduced the incidence of severe HE post-TIPS. If it occurs, shunt diameter reduction can reverse HE [
      • Fanelli F.
      • Salvatori F.M.
      • Rabuffi P.
      • Boatta E.
      • Riggio O.
      • Lucatelli P.
      • et al.
      Management of refractory hepatic encephalopathy after insertion of TIPS: long-term results of shunt reduction with hourglass-shaped balloon-expandable stent-graft.
      ]. However, the original cause for placing TIPS may reappear.
      Another important issue with TIPS relates to the desired portal pressure (PP) attained after placement of stents. Too low a pressure because of large stent diameter can lead to intractable HE, as noted above. There is a lack of consensus on whether to aim to reduce PP by 50% or below 12 mmHg. The latter is associated with more bouts of encephalopathy [
      • Chung H.H.
      • Razavi M.K.
      • Sze D.Y.
      • Frisoli J.K.
      • Kee S.T.
      • Dake M.D.
      • et al.
      Portosystemic pressure gradient during transjugular intrahepatic portosystemic shunt with Viatorr stent graft: what is the critical low threshold to avoid medically uncontrolled low pressure gradient related complications?.
      ]. It is widely used to treat post-TIPS recurrent HE as with other cases of recurrent HE, including the cases that cannot be managed by reduction of shunt diameter.

       Hepatic encephalopathy secondary to portosystemic shunts (PSSs)

      Recurrent bouts of overt HE in patients with preserved liver function consideration should lead to a search for large spontaneous PSSs. Certain types of shunts, such as splenorenal shunts, can be successfully embolized with rapid clearance of overt HE in a fraction of patients in a good liver function status, despite the risk for subsequent VB [
      • Laleman W.
      • Simon-Talero M.
      • Maleux G.
      • Perez M.
      • Ameloot K.
      • Soriano G.
      • et al.
      on behalf of the EASL-CLIF-Consortium
      Embolization of large spontaneous portosystemic shunts for refractory hepatic encephalopathy: a multi-center survey on safety and efficacy.
      ].

       Recommendations

       Discontinuation of prophylactic therapy

      There is a nearly uniform policy to continue treatment indefinitely after it has successfully reversed a bout of OHE. The concept may be that once the thresholds for OHE is reached, then patients are at high risk for recurrent episodes. This risk appears to worsen as liver function deteriorates. However, what often occurs are recurrent bouts of OHE from a well-known list of precipitating factors. If a recurrent precipitating factor can be controlled, such as recurrent infections or variceal hemorrhages, then HE recurrence may not be a risk and HE therapy can be discontinued. Even more influential on the risk for further bouts of OHE is overall liver function and body habitus. If patients recover a significant amount of liver function and muscle mass from the time they had bouts of OHE, they may well be able to stop standard HE therapy. There are very little data on this issue, but tests positive for MHE or CHE before stopping HE drug therapy will predict patients at risk for recurrent HE.

       Recommendation

       Treatment of minimal HE and covert HE

      Although it is not standard to offer therapy for MHE and CHE, studies have been performed using several modes of therapy. The majority of studies have been for less than 6 months and do not reflect the overall course of the condition. Trials span the gamut from small open-label trials to larger, randomized, controlled studies using treatments varying from probiotics, lactulose, and rifaximin. Most studies have shown an improvement in the underlying cognitive status, but the mode of diagnosis has varied considerably among studies. A minority of studies used clinically relevant endpoints. It was shown, in an open-label study [
      • Sharma P.
      • Sharma B.C.
      • Agrawal A.
      • Sarin S.K.
      Primary prophylaxis of overt hepatic encephalopathy in patients with cirrhosis: an open labeled randomized controlled trial of lactulose vs. no lactulose.
      ], that lactulose can prevent development of the first episode of OHE, but the study needs to be replicated in a larger study in a blinded fashion before firm recommendations can be made. Studies using lactulose and rifaximin have shown improvement in quality of life [
      • Prasad S.
      • Dhiman R.K.
      • Duseja A.
      • Chawla Y.K.
      • Sharma A.
      • Agarwal R.
      Lactulose improves cognitive functions and health-related quality of life in patients with cirrhosis who have minimal hepatic encephalopathy.
      ,
      • Sidhu S.S.
      • Goyal O.
      • Mishra B.P.
      • Sood A.
      • Chhina R.S.
      • Soni R.K.
      Rifaximin improves psychometric performance and health-related quality of life in patients with minimal hepatic encephalopathy (the RIME Trial).
      ] and also in driving simulator performance [
      • Bajaj J.S.
      • Heuman D.M.
      • Wade J.B.
      • Gibson D.P.
      • Saeian K.
      • Wegelin J.A.
      • et al.
      Rifaximin improves driving simulator performance in a randomized trial of patients with minimal hepatic encephalopathy.
      ]. Probiotics have also been used, but the open-label nature, varying amounts and types of organisms, and different outcomes make them difficult to recommend as therapeutic options at this time [
      • Bajaj J.S.
      • Saeian K.
      • Christensen K.M.
      • Hafeezullah M.
      • Varma R.R.
      • Franco J.
      • et al.
      Probiotic yogurt for the treatment of minimal hepatic encephalopathy.
      ,
      • Mittal V.V.
      • Sharma B.C.
      • Sharma P.
      • Sarin S.K.
      A randomized controlled trial comparing lactulose, probiotics, and L-ornithine L-aspartate in treatment of minimal hepatic encephalopathy.
      ,
      • Saji S.
      • Kumar S.
      • Thomas V.
      A randomized double blind placebo controlled trial of probiotics in minimal hepatic encephalopathy.
      ,
      • Shukla S.
      • Shukla A.
      • Mehboob S.
      • Guha S.
      Meta-analysis: the effects of gut flora modulation using prebiotics, probiotics and synbiotics on minimal hepatic encephalopathy.
      ].
      Because of the multiple methods used to define MHE and CHE, varying endpoints, short-term treatment trials, and differing agents used in trials to date, routine treatment for MHE is not recommended at this stage. Exceptions could be made on a case-by-case basis using treatments that are approved for OHE, particularly for patients with CHE and West Haven Grade I HE.

       Recommendation

       Nutrition

      Modulation of nitrogen metabolism is crucial to the management of all grades of HE, and nutritional options are relevant. Detailed recent guidelines for nutrition of patients with HE are given elsewhere [
      • Amodio P.
      • Bemeur C.
      • Butterworth R.
      • Cordoba J.
      • Kata A.
      • Montagnese S.
      • et al.
      The nutritional management of hepatic encephalopathy in patients with cirrhosis: ISHEN practice guidelines.
      ]. Malnutrition is often underdiagnosed, and approximately 75% of patients with HE suffer from moderate-to-severe protein-calorie malnutrition with loss of muscle mass and energy depots. Chronic protein restriction is detrimental because patient’s protein requirements are relatively greater than that of healthy patients and they are at risk of accelerated fasting metabolism. Malnutrition and loss of muscle bulk is a risk factor for development of HE and other cirrhosis complications. Sarcopenia has been proven to be an important negative prognostic indicator in patients with cirrhosis [
      • Montano-Loza A.J.
      • Meza-Junco J.
      • Prado C.M.
      • Lieffers J.R.
      • Baracos V.E.
      • Bain V.G.
      • et al.
      Muscle wasting is associated with mortality in patients with cirrhosis.
      ,
      • Tandon P.
      • Ney M.
      • Irwin I.
      • Ma M.M.
      • Gramlich L.
      • Bain V.G.
      • et al.
      Severe muscle depletion in patients on the liver transplant wait list: its prevalence and independent prognostic value.
      ]. All HE patients should undergo an assessment of nutritional status by taking a good dietary history, with anthropometric data and muscle strength measurement as practical, useful measures of nutritional status. In the undressed patient, particular attention is paid to the muscle structures around the shoulders and gluteal muscles. Pitfalls are water retention and obesity. Although body mass index is rarely helpful, the height-creatinine ratio may be useful, as well as the bioimpedance technique. More advanced techniques, such as dual-energy X-ray absorptiometry/CT/MR, are rarely useful for clinical purposes. The patient should undergo a structured dietary assessment, preferably by a dietician, or other specially trained staff. The majority of HE patients will fulfil criteria for nutritional therapy. The therapy is refeeding by moderate hyperalimentation, as indicated below. Small meals evenly distributed throughout the day and a late-night snack [
      • Tsien C.D.
      • McCullough A.J.
      • Dasarathy S.
      Late evening snack: exploiting a period of anabolic opportunity in cirrhosis.
      ] should be encouraged, with avoidance of fasting. Glucose may be the most readily available calorie source, but should not be utilized as the only nutrition. Hyperalimentation should be given orally to patients that can cooperate, by gastric tube to patients who cannot take the required amount, and parenterally to other patients. The nutrition therapy should be initiated without delay and monitored during maintenance visits. The use of a multivitamin is generally recommended, although there are no firm data on the benefits of vitamin and mineral supplementation. Specific micronutrient replacement is given if there are confirmed measured losses, and zinc supplementation is considered when treating HE. If Wernicke’s is suspected, large doses of thiamine should be given parenterally and before any glucose administration. Administration of large amounts of nonsaline fluids should be adjusted so as to avoid induction of hyponatremia, particularly in patients with advanced cirrhosis. If severe hyponatremia is corrected, this should be done slowly.
      There is consensus that low-protein nutrition should be avoided for patients with HE. Some degree of protein restriction may be inevitable in the first few days of OHE treatment, but should not be prolonged. Substitution of milk-based or vegetable protein or supplementing with BCAAs is preferable to reduction of total protein intake. Oral BCAA-enriched nutritional formulation may be used to treat HE and generally improves the nutritional status of patients with cirrhosis [
      • Ndraha S.
      • Hasan I.
      • Simadibrata M.
      The effect of L-ornithine L-aspartate and branch chain amino acids on encephalopathy and nutritional status in liver cirrhosis with malnutrition.
      ], but IV BCAA for an episode of HE has no effect [
      • Naylor C.D.
      • O’Rourke K.
      • Detsky A.S.
      • Baker J.P.
      Parenteral nutrition with branched-chain amino acids in hepatic encephalopathy. A meta-analysis.
      ]. The studies on the effect of oral BCAA have been more encouraging [
      • Marchesini G.
      • Bianchi G.
      • Merli M.
      • Amodio P.
      • Panella C.
      • Loguercio C.
      • et al.
      Nutritional supplementation with branched-chain amino acids in advanced cirrhosis: a double-blind, randomized trial.
      ,
      • Marchesini G.
      • Marzocchi R.
      • Noia M.
      • Bianchi G.
      Branched-chain amino acid supplementation in patients with liver diseases.
      ] and confirmed by a recent meta-analysis of 11 trials [
      • Gluud L.L.
      • Dam G.
      • Borre M.
      • Les I.
      • Cordoba J.
      • Marchesini G.
      • et al.
      Oral branched chain amino acids have a beneficial effect on manifestations of hepatic encephalopathy in a systematic review with meta-analyses of randomized controlled trials.
      ]. Ultimately, the effects of these amino acids may turn out to have more important effects on promotion of maintenance of lean body mass than a direct effect on HE.

       Recommendations

       Liver transplantation (LT)

      Liver Transplantation remains the only treatment option for HE that does not improve on any other treatment, but is not without its risks. The management of these potential transplant candidates as practiced in the United States has been published elsewhere [
      • Martin P.
      • DiMartini A.
      • Feng S.
      • Brown Jr., R.
      • Fallon M.
      Evaluation for liver transplantation in adults: 2013 Practice Guideline by the American Association for the Study of Liver Diseases and the American Society of Transplantation.
      ,
      • Lucey M.R.
      • Terrault N.
      • Ojo L.
      • Hay J.E.
      • Neuberger J.
      • Blumberg E.
      • et al.
      Long-term management of the successful adult liver transplant: 2012 Practice Guideline by the American Association for the Study of Liver Diseases and the American Society of Transplantation.
      ], and European guidelines are under way. Hepatic encephalopathy by itself is not considered an indication for LT unless associated with poor liver function. However, cases do occur where HE severely compromises the patient’s quality of life and cannot be improved despite maximal medical therapy and who may be LT candidates despite otherwise good liver status. Large PSSs may cause neurological disturbances and persistent HE, even after LT. Therefore, shunts should be identified and embolization considered before or during transplantation [
      • Herrero J.I.
      • Bilbao J.I.
      • Diaz M.L.
      • Alegre F.
      • Inarrairaegui M.
      • Pardo F.
      • et al.
      Hepatic encephalopathy after liver transplantation in a patient with a normally functioning graft: treatment with embolization of portosystemic collaterals.
      ]. Also, during the transplant workup, severe hyponatremia should be corrected slowly.
      Hepatic encephalopathy should improve after transplant, whereas neurodegenerative disorders will worsen. Therefore, it is important to distinguish HE from other causes of mental impairment, such as Alzheimer’s disease and small-vessel cerebrovascular disease. Magnetic resonance imaging and spectroscopy of the brain should be conducted, and the patient should be evaluated by an expert in neuropsychology and neurodegenerative diseases [
      • Chavarria L.
      • Alonso J.
      • García-Martínez R.
      • Simón-Talero M.
      • Ventura-Cots M.
      • Ramírez C.
      • et al.
      Brain magnetic resonance spectroscopy in episodic hepatic encephalopathy.
      ]. The patient, caregivers, and health professionals should be aware that transplantation may induce brain function impairment and that not all manifestations of HE are fully reversible by transplantation [
      • Garcia-Martinez R.
      • Rovira A.
      • Alonso J.
      • Jacas C.
      • Simón-Talero M.
      • Chavarria L.
      • et al.
      Hepatic encephalopathy is associated with posttransplant cognitive function and brain volume.
      ].
      One difficult and not uncommon problem is the development of a confusional syndrome in the postoperative period. The search of the cause is often difficult, and the problem may have multiple origins. Patients with alcoholic liver disease (ALD) and those with recurrent HE before transplantation are at higher risk. Toxic effects of immunosuppressant drugs are a frequent cause, usually associated with tremor and elevated levels in blood. Other adverse cerebral effects of drugs may be difficult to diagnose. Confusion associated with fever requires a diligent, systematic search for bacterial or viral causes (e.g., cytomegalovirus). Multiple causative factors are not unusual, and the patient’s problem should be approached from a broad clinical view [
      • Amodio P.
      • Biancardi A.
      • Montagnese S.
      • Angeli P.
      • Iannizzi P.
      • Cillo U.
      • et al.
      Neurological complications after orthotopic liver transplantation.
      ].

       Economic/cost implications

      As outlined under epidemiology, the burden of HE is rapidly increasing and more cases of HE will be encountered, with substantial direct costs being attributed to hospitalizations for HE and to indirect costs. The patients with HE hospitalized in the United States in 2003 generated charges of approximately US$ 1 billion [
      • Stepanova M.
      • Mishra A.
      • Venkatesan C.
      • Younossi Z.M.
      In-hospital mortality and economic burden associated with hepatic encephalopathy in the United States from 2005 to 2009.
      ,
      • Poordad F.F.
      Review article: the burden of hepatic encephalopathy.
      ]. Resource utilization for this group of patients is also increasing as a result of longer lengths of stay and more complex and expensive hospital efforts, as well as a reported in-patient mortality of 15%. There are no directly comparable EU cost data, but by inference from epidemiological data, the event rate should be approximately the same and the costs comparable, differences between U.S. and EU hospital financing notwithstanding. These costs are an underestimate, because out-patient care, disability and lost productivity, and the negative effect on the patient’s family or support network were not quantified [
      • Bajaj J.S.
      • Wade J.B.
      • Gibson D.P.
      • Heuman D.M.
      • Thacker L.R.
      • Sterling R.K.
      • et al.
      The multi-dimensional burden of cirrhosis and hepatic encephalopathy on patients and caregivers.
      ].
      The cost of medications is very variable to include in analyses because it varies widely from country to country and are usually determined by what the pharmaceutical companies believe the market can sustain. Regarding the beneficial effects of rifaximin, cost-effective analyses based on current drug prices favor treatments that are lactulose based [
      • Huang E.
      • Esrailian E.
      • Spiegel B.M.
      The cost-effectiveness and budget impact of competing therapies in hepatic encephalopathy—a decision analysis.
      ,
      • Neff G.W.
      • Kemmer N.
      • Zacharias V.C.
      • Kaiser T.
      • Duncan C.
      • McHenry R.
      • et al.
      Analysis of hospitalizations comparing rifaximin vs. lactulose in the management of hepatic encephalopathy.
      ], as do analyses of accidents, deaths/morbidity, and time off from work [
      • Bajaj J.S.
      • Pinkerton S.D.
      • Sanyal A.J.
      • Heuman D.M.
      Diagnosis and treatment of minimal hepatic encephalopathy to prevent motor vehicle accidents: a cost-effectiveness analysis.
      ] in patients with MHE or CHE. Therefore, until the costs of other medications fall, lactulose continues to be the least expensive, most cost-effective treatment.

      Alternative causes of altered mental status

       Disorders to be considered

      The neurological manifestations of HE are nonspecific. Therefore, concomitant disorders have to be considered as an additional source of central nervous system dysfunction in any patient with CLD. Most important are renal dysfunction, hyponatremia, diabetes mellitus (DM), sepsis, and thiamine deficiency (Wernicke’s encephalopathy); noteworthy also is intracranial bleeding (chronic subdural hematoma and parenchymal bleeding).

       Interaction between concomitant disorders and liver disease with regard to brain function

      Hyponatremia is an independent risk factor for development of HE in patients with cirrhosis [
      • Guevara M.
      • Baccaro M.E.
      • Torre A.
      • Gómez-Ansón B.
      • Rìos J.
      • Torres F.
      • et al.
      Hyponatremia is a risk factor of hepatic encephalopathy in patients with cirrhosis: a prospective study with time-dependent analysis.
      ,
      • Guevara M.
      • Baccaro M.E.
      • Ríos J.
      • Martín-Llahí M.
      • Uriz J.
      • Ruiz del Arbol L.
      • et al.
      Risk factors for hepatic encephalopathy in patients with cirrhosis and refractory ascites: relevance of serum sodium concentration.
      ]. The incidence of HE increases [
      • Angeli P.
      • Wong F.
      • Watson H.
      • Gines P.
      Hyponatremia in cirrhosis: results of a patient population survey.
      ] and the response rate to lactulose therapy decreases [
      • Sharma P.
      • Sharma B.C.
      • Sarin S.K.
      Predictors of nonresponse to lactulose for minimal hepatic encephalopathy in patients with cirrhosis.
      ] with decreasing serum sodium concentrations.
      Diabetes mellitus has been suggested as a risk factor for development of HE, especially in patients with hepatitis C virus (HCV) cirrhosis [
      • Sigal S.H.
      • Stanca C.M.
      • Kontorinis N.
      • Bodian C.
      • Ryan E.
      Diabetes mellitus is associated with hepatic encephalopathy in patients with HCV cirrhosis.
      ], but the relationship may also be observed in other cirrhosis etiologies [
      • Kalaitzakis E.
      • Olsson R.
      • Henfridsson P.
      • Hugosson I.
      • Bengtsson M.
      • Jalan R.
      • et al.
      Malnutrition and diabetes mellitus are related to hepatic encephalopathy in patients with liver cirrhosis.
      ].
      An increased risk to develop HE has also been shown in patients with cirrhosis with renal dysfunction [
      • Kalaitzakis E.
      • Bjornsson E.
      Renal function and cognitive impairment in patients with liver cirrhosis.
      ], independent of the severity of cirrhosis.
      Neurological symptoms are observed in 21%–33% of patients with cirrhosis with sepsis and in 60%–68% of those with septic shock [
      • Gustot T.
      • Durand F.
      • Lebrec D.
      • Vincent J.L.
      • Moreau R.
      Severe sepsis in cirrhosis.
      ]. Patients with cirrhosis do not differ from patients without cirrhosis regarding their risk to develop brain dysfunction with sepsis [
      • Young G.B.
      • Bolton C.F.
      • Austin T.W.
      • Archibald Y.M.
      • Gonder J.
      • Wells G.A.
      The encephalopathy associated with septic illness.
      ], although it is assumed that systemic inflammation and hyperammonemia act synergistically with regard to the development of HE.
      Thiamine deficiency predominantly occurs in patients with ALD, but may also occur as a consequence of malnutrition in end-stage cirrhosis of any cause. The cerebral symptoms disorientation, alteration of consciousness, ataxia, and dysarthria cannot be differentiated as being the result of thiamine deficiency or hyperammonemia by clinical examination [
      • Butterworth R.F.
      Thiamine deficiency-related brain dysfunction in chronic liver failure.
      ]. In any case of doubt, thiamine should be given IV before glucose-containing solutions.

       Effect of the etiology of the liver disease upon brain function

      Data upon the effect of the underlying liver disease on brain function are sparse, except for alcoholism and hepatitis C. Rare, but difficult, cases may be the result of Wilson’s disease.
      Even patients with alcohol disorder and no clinical disease have been shown to exhibit deficits in episodic memory [
      • Pitel A.L.
      • Beaunieux H.
      • Witkowski T.
      • Vabret F.
      • Guillery-Girard B.
      • Quinette P.
      • et al.
      Genuine episodic memory deficits and executive dysfunctions in alcoholic subjects early in abstinence.
      ], working memory and executive functions [
      • Noel X.
      • Van der Linden M.
      • Schmidt N.
      • Sferrazza R.
      • Hanak C.
      • Le Bon O.
      • et al.
      Supervisory attentional system in nonamnestic alcoholic men.
      ], visuoconstruction abilities [
      • Dawson L.K.
      • Grant I.
      Alcoholics’ initial organizational and problem-solving skills predict learning and memory performance on the Rey-Osterrieth Complex Figure.
      ], and upper- and lower-limb motor skills [
      • Sullivan E.V.
      • Rosenbloom M.J.
      • Pfefferbaum A.
      Pattern of motor and cognitive deficits in detoxified alcoholic men.
      ]. The cognitive dysfunction is more pronounced in those patients with alcohol disorder who are at risk of Wernicke’s encephalopathy as a result of malnutrition or already show signs of the problem [
      • Pitel A.L.
      • Zahr N.M.
      • Jackson K.
      • Sassoon S.A.
      • Rosenbloom M.J.
      • Pfefferbaum A.
      • et al.
      Signs of preclinical Wernicke’s encephalopathy and thiamine levels as predictors of neuropsychological deficits in alcoholism without Korsakoff’s syndrome.
      ]. Thus, it remains unclear whether the disturbance of brain function in patients with ALD is the result of HE, alcohol toxicity, or thiamine deficiency.
      There is mounting evidence that HCV is present and replicates within the brain [
      • Laskus T.
      • Radkowski M.
      • Bednarska A.
      • Wilkinson J.
      • Adair D.
      • Nowicki M.
      • et al.
      Detection and analysis of hepatitis C virus sequences in cerebrospinal fluid.
      ,
      • Forton D.M.
      • Karayianni P.
      • Mahmud N.
      • Taylor-Robinson S.D.
      • Thomas H.C.
      Identification of unique hepatitis C virus quasispecies in the central nervous system and comparative analysis of internal translational efficiency of brain, liver, and serum variants.
      ,
      • Fishman S.L.
      • Murray J.M.
      • Eng F.J.
      • Walewski J.L.
      • Morgello S.
      • Branch A.D.
      Molecular and bioinformatic evidence of hepatitis C virus evolution in brain.
      ,
      • Wilkinson J.
      • Radkowski M.
      • Laskus T.
      Hepatitis C virus neuroinvasion: identification of infected cells.
      ]. Approximately half of HCV patients suffer chronic fatigue irrespective of the grade of their liver disease [
      • Poynard T.
      • Cacoub P.
      • Ratziu V.
      • Myers R.P.
      • Dezailles M.H.
      • Mercadier A.
      • et al.
      For the Multivirc Group. Fatigue in patients with chronic hepatitis C.
      ,
      • Hassoun Z.
      • Willems B.
      • Deslauriers J.
      • Nguyen B.N.
      • Huet P.M.
      Assessment of fatigue in patients with chronic hepatitis C using the fatigue impact scale.
      ], and even patients with only mild liver disease display cognitive dysfunction [
      • Forton D.M.
      • Thomas H.C.
      • Murphy C.A.
      • Allsop J.M.
      • Foster G.R.
      • Main J.
      • et al.
      Hepatitis C and cognitive impairment in a cohort of patients with mild liver disease.
      ,
      • Weissenborn K.
      • Krause J.
      • Bokemeyer M.
      • Hecker H.
      • Schüler A.
      • Ennen J.C.
      • et al.
      Hepatitis C virus infection affects the brain—evidence from psychometric studies and magnetic resonance spectroscopy.
      ], involving verbal learning, attention, executive function, and memory. Likewise, patients with primary biliary cirrhosis and primary sclerosing cholangitis may have severe fatigue and impairment of attention, concentration, and psychomotor function irrespective of the grade of liver disease [
      • Tarter R.E.
      • Hegedus A.M.
      • Van Thiel D.H.
      • Edwards N.
      • Schade R.R.
      Neurobehavioral correlates of cholestatic and hepatocellular disease: dif ferentiation according to disease specific characteristics and severity of the identified cerebral dysfunction.
      ,
      • Tarter R.E.
      • Hays A.L.
      • Carra J.
      • Edwards K.L.
      • Van Thiel D.H.
      Sjogren’s syndrome. Its contribution to neuropsychiatric syndrome in patients with primary biliary cirrhosis.
      ,
      • Newton J.L.
      • Hollingsworth K.G.
      • Taylor R.
      • El-Sharkawy A.M.
      • Khan Z.U.
      • Pearce R.
      • et al.
      Cognitive impairment in primary biliary cirrhosis: symptom impact and potential etiology.
      ,
      • Jacoby A.
      • Rannard A.
      • Buck D.
      • Bhala N.
      • Newton J.L.
      • James O.F.W.
      • et al.
      Development, validation and evaluation of the PBC-40, a disease specific health related quality of life measure for primary biliary cirrhosis.
      ,
      • Newton J.L.
      • Bhala N.
      • Burt J.
      • Jones D.E.J.
      Characterisation of the associations and impact of symptoms in primary biliary cirrhosis using a disease specific quality of life measure.
      ,
      • Newton J.L.
      • Gibson J.G.
      • Tomlinson M.
      • Wilton K.
      • Jones D.E.J.
      Fatigue in primary biliary cirrhosis is associated with excessive daytime somnolence.
      ].

       Diagnostic measures to differentiate between HE and cerebral dysfunction resulting from other causes

      Because HE shares symptoms with all concomitant disorders and underlying diseases, it is difficult in the individual case to differentiate between the effects of HE and those resulting from other causes. In some cases, the time course and response to therapy may be the best support of HE. As mentioned, a normal blood ammonia level in a patient suspected of HE calls for consideration. None of the diagnostic measures used at present has been evaluated for their ability to differentiate between HE and other causes of brain dysfunction. The EEG would not be altered by DM or alcohol disorders, but may show changes similar to those with HE in cases of renal dysfunction, hyponatremia, or septic encephalopathy. Psychometric tests are able to detect functional deficits, but are unable to differentiate between different causes for these deficits. Brain imaging methods have been evaluated for their use in diagnosing HE, but the results are disappointing. Nevertheless, brain imaging should be done in every patient with CLD and unexplained alteration of brain function to exclude structural lesions. In rare cases, reversibility by flumazenil may be useful.

      Follow-up

      After a hospital admission for HE, the following issues should be addressed.

       Discharge from hospital

      • (1)
        The medical team should confirm the neurological status before discharge and judge to what extent the patient’s neurological deficits could be attributable to HE, or to other neurological comorbidities, for appropriate discharge planning. They should inform caregivers that the neurological status may change once the acute illness has settled and that requirement for medication could change.
      • (2)
        Precipitating and risk factors for development of HE should be recognized. Future clinical management should be planned according to (1) potential for improvement of liver function (e.g., acute alcoholic hepatitis, autoimmune hepatitis, and hepatitis B), (2) presence of large portosystemic shunts (which may be suitable for occlusion), and (3) characteristics of precipitating factors (e.g., prevention of infection, avoidance of recurrent GI bleeding, diuretics, or constipation).
      • (3)
        Out-patient postdischarge consultations should be planned to adjust treatment and prevent the reappearance of precipitating factors. Close liaison should be made with the patient’s family, the general practitioner, and other caregivers in the primary health service, so that all parties involved understand how to manage HE in the specific patient and prevent repeated hospitalizations.

       Preventive care after discharge

      • (1)
        Education of patients and relatives should include (1) effects of medication (lactulose, rifaximin, and so on) and the potential side effects (e.g., diarrhea), (2) importance of adherence, (3) early signs of recurring HE, and (4) actions to be taken if recurrence (e.g., anticonstipation measures for mild recurrence and referral to general practitioner or hospital if HE with fever).
      • (2)
        Prevention of recurrence: the underlying liver pathology may improve with time, nutrition, or specific measures, but usually patients who have developed OHE have advanced liver failure without much hope for functional improvements and are often potential LT candidates. Managing the complications of cirrhosis (e.g., spontaneous bacterial peritonitis and GI bleeding) should be instituted according to available guidelines. Pharmacological secondary prevention is mentioned above.
      • (3)
        Monitoring neurological manifestations is necessary in patients with persisting HE to adjust treatment and in patients with previous HE to investigate the presence and degree of MHE or CHE or signs of recurring HE. The cognitive assessment depends on the available normative data and local resources. The motor assessment should include evaluation of gait and walking and consider the risk of falls.
      • (4)
        The socioeconomic implications of persisting HE or MHE or CHE may be very profound. They include a decline in work performance, impairment in quality of life, and increase in the risk of accidents. These patients often require economic support and extensive care from the public social support system and may include their relatives. All these issues should be incorporated into the follow-up plan.
      • (5)
        Treatment endpoints depend on the monitoring used and the specialist clinic, but at least they have to cover two aspects: (1) cognitive performance (improvement in one accepted test as a minimum) and (2) daily life autonomy (basic and operational abilities).
      • (6)
        Nutritional aspects: weight loss with sarcopenia may worsen HE, and, accordingly, the nutritional priority is to provide enough protein and energy to favor a positive nitrogen balance and increase in muscle mass, as recommended above.
      • (7)
        Portosystemic shunt: occlusion of a dominant shunt may improve HE in patients with recurring HE and good liver function [
        • Laleman W.
        • Simon-Talero M.
        • Maleux G.
        • Perez M.
        • Ameloot K.
        • Soriano G.
        • et al.
        on behalf of the EASL-CLIF-Consortium
        Embolization of large spontaneous portosystemic shunts for refractory hepatic encephalopathy: a multi-center survey on safety and efficacy.
        ]. Because the current experience is limited, the risks and benefits must be weighed before employing this strategy.

      Suggestions for future research

      This section deals with research into the management of HE. However, such research should always be based on research into the pathophysiology of HE. It is necessary to gain more insight into which liver functions are responsible for maintenance of cerebral functions, which alterations in intestinal function and microbiota make failure of these liver functions critical, which brain functions are particularly vulnerable to the combined effects of the aforementioned events, and, finally, which factors outside this axis that result in the emergence of HE (e.g., inflammation, endocrine settings, or malnutrition). Therefore, the research fields into pathophysiology and clinical management should remain in close contact. Such collaboration should result in new causal and symptomatic treatment modalities that need and motivate clinical trials.
      There is a severe and unmet need for controlled clinical trials on treatment effects on all the different forms of HE. Decisive clinical studies are few, although the number of patients and their resource utilization is high. There are no data on which factors and patients represent the higher costs, and research is needed to examine the effect of specific cirrhosis-related complications. At present, there is an insufficient basis for allocating resources and establishing priority policies regarding management of HE. Many drugs that were assessed for HE several decades ago were studied following a standard of care that, at present, is obsolete. Any study of treatment for HE should be reassessed or repeated using the current standard of care. It is critical to develop protocols to identify precipitating factors and ACLF. The benefit of recently assessed drugs is concentrated in the prevention of recurrence, and there is a large need for trials on episodic HE.
      There is also an unmet need for research into diagnostic methods that is necessary to form a basis for clinical trials. The diagnosis of MHE and CHE has received enormous interest, but it is still not possible to compare results among studies and the precision should be improved. It may be useful to develop, validate, and implement HE scales that combine the degree of functional liver failure and PSS with more than one psychometric method.
      One important area of uncertainty is whether the term CHE, which was introduced to expand MHE toward grade I of oriented patients, is informative and clinically valuable. This needs to be evaluated by a data-driven approach. Likewise, the distinction between isolated liver failure and ACLF-associated HE should be evaluated by independent data.
      A closer scientific collaboration between clinical hepatologists and dedicated brain researchers, including functional brain imaging experts, is needed. Likewise, neuropsychologists and psychiatrists are needed to clarify the broad spectrum of neuropsychiatric symptoms that can be observed in patients with liver disease. Syndrome diagnoses should be more precisely classified and transformed into classifiable entities based on pathophysiology and responding to the requirements of clinical hepatology practice and research.
      Future studies should fill our gaps in knowledge. They should be focused on assessing the effects of HE on individuals and society, how to use diagnostic tools appropriately, and define the therapeutic goals in each clinical scenario (Table 7).
      Table 7Suggested areas of future research in HE.

       Recommendations on future research in HE

      The existing literature suffers from a lack of standardization, and this heterogeneity makes pooling of data difficult or meaningless. Recommendations to promote consistency across the field have been published by ISHEN [
      • Bajaj J.S.
      • Cordoba J.
      • Mullen K.D.
      • Amodio P.
      • Shawcross D.L.
      • Butterrworth R.F.
      • et al.
      Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
      ]. Following is a synopsis of the recommendations.

       Trials in patients with episodic OHE

      • (1)
        Patients who are not expected to survive the hospitalization, who are terminally ill or have ACLF should be excluded.
      • (2)
        A detailed standard-of-care algorithm must be agreed upon a priori and must be instituted and monitored diligently throughout the trial.
      • (3)
        Patients should not be entered into trials until after the institution of optimal standard-of-care therapy and only if their mental state abnormalities persist.
      • (4)
        Provided the optimal standard of care is instituted and maintained, the treatment trial can be initiated earlier if they include a placebo comparator; this would allow an evaluation of the trial treatment as an adjuvant to standard therapy.
      • (5)
        Large-scale, multicenter treatment trials should be evaluated using robust clinical outcomes, such as in-hospital and remote survival, liver-related and total deaths, completeness and speed of recovery from HE, number of days in intensive care, total length of hospital stay, quality-of-life measures, and associated costs. Markers for HE, such as psycho metric testing, can be employed if standardized and validated tools are available in all centers. Individual centers can utilize additional, accessible, validated markers if they choose.
      • (6)
        Proof-of-concept trials will additionally be monitored using tools that best relate to the endpoints anticipated or expected; this may involve use of neural imaging or measurement of specific biomarkers.

       Trials in patients with MHE or CHE

      Trials in this population should be randomized and placebo controlled.
      • (1)
        Patients receiving treatment for OHE or those with previous episodes of OHE should be excluded.
      • (2)
        In single-center or proof-of-concept studies, investigators may use tests for assessing the severity of HE with which they are familiar, provided that normative reference data are available and the tests have been validated for use in this patient population.
      • (3)
        Further information is needed on the interchangeability and standardization of tests to assess the severity of HE for use in multicenter trials. As an interim, two or more of the current validated tests should be used and applied uniformly across centers.

      Conflict of interest

      Dr. Wong consults, advises, and received grants from Gilead. He consults and advises Roche. He advises and received grants from Vertex. Dr. Ferenci advises Ocera and Salix. Dr. Bajaj consults and received grants from Otsuka and Grifols. He consults for Salix. Dr. Mullen is on the speakers’ bureau for Salix and Abbott.

      References

        • Guyatt G.H.
        • Oxman A.D.
        • Vist G.E.
        • Kunz R.
        • Falck-Ytter Y.
        • Alono-Coello P.
        • et al.
        GRADE: an emerging consensus on rating quality of evidence and strength of recommendations.
        BMJ. 2008; 336: 924-926
        • Rakoski M.O.
        • McCammon R.J.
        • Piette J.D.
        • Iwashyna T.J.
        • Marrero J.A.
        • Lok A.S.
        • et al.
        Burden of cirrhosis on older Americans and their families: analysis of the health and retirement study.
        Hepatology. 2012; 55: 184-191
        • Sherlock S.
        • Summerskill W.H.J.
        • White L.P.
        • Phear E.A.
        Portal-systemic encephalopathy. Neurological complications of liver disease.
        Lancet. 1954; 264: 453-457
        • Fazekas J.E.
        • Ticktin H.E.
        • Shea J.G.
        Effects of L-arginine on hepatic encephalopathy.
        Am J Med Sci. 1957; 234: 462-467
        • Kaplan P.W.
        • Rossetti A.O.
        EEG patterns and imaging correlations in encephalopathy: encephalopathy part II.
        J Clin Neurophysiol. 2011; 28: 233-251
        • Conn H.O.
        Hepatic encephalopathy.
        in: Schiff L. Schiff E.R. Diseases of the liver. 7th ed. Lippincott, Philadelphia, PA1993: 1036-1060
        • D’Amico G.
        • Morabito A.
        • Pagliaro L.
        • Marubini E.
        Survival and prognostic indicators in compensated and decompensated cirrhosis.
        Dig Dis Sci. 1986; 31: 468-475
        • Ding A.
        • Lee A.
        • Callender M.
        • Loughrey M.
        • Quah S.P.
        • Dinsmore W.W.
        Hepatic encephalopathy as an unusual late complication of transjugular intrahepatic portosystemic shunt insertion for non-cirrhotic portal hypertension caused by nodular regenerative hyperplasia in an HIV-positive patient on highly active antiretroviral therapy.
        Int J STD AIDS. 2010; 21: 71-72
        • Ito T.
        • Ikeda N.
        • Watanabe A.
        • Sue K.
        • Kakio T.
        • Mimura H.
        • et al.
        Obliteration of portal systemic shunts as therapy for hepatic encephalopathy in patients with non-cirrhotic portal hypertension.
        Gastroenterol Jpn. 1992; 27: 759-764
        • Ferenci P.
        • Lockwood A.
        • Mullen K.
        • Tarter R.
        • Weissenborn K.
        • Blei A.T.
        Hepatic encephalopathy—definition, nomenclature, diagnosis, and quantification: final report of the working party at the 11th World Congresses of Gastroenterology, Vienna, 1998.
        Hepatology. 2002; 35: 716-721
        • Cordoba J.
        New assessment of hepatic encephalopathy.
        J Hepatol. 2011; 54: 1030-1040
        • Rikkers L.
        • Jenko P.
        • Rudman D.
        • Freides D.
        Subclinical hepatic encephalopathy: detection, prevalence, and relationship to nitrogen metabolism.
        Gastroenterology. 1978; 75: 462-469
        • Del Piccolo F.
        • Sacerdoti D.
        • Amodio P.
        • Bombonato G.
        • Bolognesi M.
        • Mapelli D.
        • et al.
        Central nervous system alterations in liver cirrhosis: the role of portal-systemic shunt and portal hypoperfusion.
        Metab Brain Dis. 2002; 17: 347-358
        • Bajaj J.S.
        • Schubert C.M.
        • Heuman D.M.
        • Wade J.B.
        • Gibson D.P.
        • Topaz A.
        • et al.
        Persistence of cognitive impairment after resolution of overt hepatic encephalopathy.
        Gastroenterology. 2010; 138: 2332-2340
        • Riggio O.
        • Ridola L.
        • Pasquale C.
        • Nardelli S.
        • Pentassuglio I.
        • Moscucci F.
        • et al.
        Evidence of persistent cognitive impairment after resolution of overt hepatic encephalopathy.
        Clin Gastroenterol Hepatol. 2011; 9: 181-183
        • Saunders J.B.
        • Walters J.R.F.
        • Davies P.
        • Paton A.
        A 20-year prospective study of cirrhosis.
        BMJ. 1981; 282: 263-266
        • Romero-Gomez M.
        • Boza F.
        • Garcia-Valdecasas M.S.
        • et al.
        Subclinical hepatic encephalopathy predicts the development of overt hepatic encephalopathy.
        Am J Gastroenterol. 2001; 96: 2718-2723
        • Jepsen P.
        • Ott P.
        • Andersen P.K.
        • Sørensen H.T.
        • Vilstrup H.
        The clinical course of alcoholic liver cirrhosis: a Danish population-based cohort study.
        Hepatology. 2010; 51: 1675-1682
        • Coltorti M.
        • Del Vecchio-Blanco C.
        • Caporaso N.
        • Gallo C.
        • Castellano L.
        Liver cirrhosis in Italy. A multicentre study on presenting modalities and the impact on health care resources. National Project on Liver Cirrhosis Group.
        Ital J Gastroenterol. 1991; 23: 42-48
        • Papatheodoridis G.V.
        • Goulis J.
        • Leandro G.
        • Patch D.
        • Burroughs A.K.
        Transjugular intrahepatic portosystemic shunt compared with endoscopic treatment for prevention of variceal rebleeding: a meta-analysis.
        Hepatology. 1999; 30: 612-622
        • Nolte W.
        • Wiltfang J.
        • Schindler C.
        • Münke H.
        • Unterberg K.
        • Zumhasch U.
        • et al.
        Portosystemic hepatic encephalopathy after transjugular intrahepatic portosystemic shunt in patients with cirrhosis: clinical, laboratory, psychometric, and electroencephalographic investigations.
        Hepatology. 1998; 28: 1215-1225
        • Amodio P.
        • Del Piccolo F.
        • Pettenò E.
        • Mapelli D.
        • Angeli P.
        • Iemmolo R.
        • et al.
        Prevalence and prognostic value of quantified electroencephalogram (EEG) alterations in cirrhotic patients.
        J Hepatol. 2001; 35: 37-45
        • Groeneweg M.
        • Moerland W.
        • Quero J.C.
        • Krabbe P.F.
        • Schalm S.W.
        Screening of subclinical hepatic encephalopathy.
        J Hepatol. 2000; 32: 748-753
        • Saxena N.
        • Bhatia M.
        • Joshi Y.K.
        • Garg P.K.
        • Tandon R.K.
        Auditory P300 event-related potentials and number connection test for evaluation of subclinical hepatic encephalopathy in patients with cirrhosis of the liver: a follow-up study.
        J Gastroenterol Hepatol. 2001; 16: 322-327
        • Schomerus H.
        • Hamster W.
        Quality of life in cirrhotics with minimal hepatic encephalopathy.
        Metab Brain Dis. 2001; 16: 37-41
        • Sharma P.
        • Sharma B.C.
        • Puri V.
        • Sarin S.K.
        Critical flicker frequency: diagnostic tool for minimal hepatic encephalopathy.
        J Hepatol. 2007; 47: 67-73
        • Bajaj J.S.
        Management options for minimal hepatic encephalopathy.
        Expert Rev Gastroenterol Hepatol. 2008; 2: 785-790
        • Bustamante J.
        • Rimola A.
        • Ventura P.J.
        • Navasa M.
        • Cirera I.
        • Reggiardo V.
        • et al.
        Prognostic significance of hepatic encephalopathy in patients with cirrhosis.
        J Hepatol. 1999; 30: 890-895
        • Hartmann I.J.
        • Groeneweg M.
        • Quero J.C.
        • Beijeman S.J.
        • de Man R.A.
        • Hop W.C.
        • et al.
        The prognostic significance of subclinical hepatic encephalopathy.
        Am J Gastroenterol. 2000; 95: 2029-2034
        • Gentilini P.
        • Laffi G.
        • La Villa G.
        • Romanelli R.G.
        • Buzzelli G.
        • Casini-Raggi V.
        • et al.
        Long course and prognostic factors of virus-induced cirrhosis of the liver.
        Am J Gastroenterol. 1997; 92: 66-72
        • Benvegnoù L.
        • Gios M.
        • Boccato S.
        • Alberti A.
        Natural history of compensated viral cirrhosis: a prospective study on the incidence and hierarchy of major complications.
        Gut. 2004; 53: 744-749
        • Watson H.
        • Jepsen P.
        • Wong F.
        • Gines P.
        • Cordoba J.
        • Vilstrup H.
        Satavaptan treatment for ascites in patients with cirrhosis: a meta-analysis of effect on hepatic encephalopathy development.
        Metab Brain Dis. 2013; 28: 301-305
        • Sharma B.C.
        • Sharma P.
        • Agrawal A.
        • Sarin S.K.
        Secondary prophylaxis of hepatic encephalopathy: an open-label randomized controlled trial of lactulose vs. placebo.
        Gastroenterology. 2009; 137 ([891.e1]): 885-891
        • Prasad S.
        • Dhiman R.K.
        • Duseja A.
        • Chawla Y.K.
        • Sharma A.
        • Agarwal R.
        Lactulose improves cognitive functions and health-related quality of life in patients with cirrhosis who have minimal hepatic encephalopathy.
        Hepatology. 2007; 45: 549-559
        • Amodio P.
        • Pellegrini A.
        • Ubiali E.
        • Mathy I.
        • Piccolo F.D.
        • Orsato R.
        • et al.
        The EEG assessment of low-grade hepatic encephalopathy: comparison of an artificial neural network-expert system (ANNES) based evaluation with visual EEG readings and EEG spectral analysis.
        Clin Neurophysiol. 2006; 117: 2243-2251
        • Boyer T.D.
        • Haskal Z.J.
        The role of transjugular intrahepatic portosystemic shunt (TIPS) in the management of portal hypertension: update 2009.
        Hepatology. 2010; 51: 306
        • Riggio O.
        • Angeloni S.
        • Salvatori F.M.
        • De S.A.
        • Cerini F.
        • Farcomeni A.
        • et al.
        Incidence, natural history, and risk factors of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt with polytetrafluoroethylene-covered stent grafts.
        Am J Gastroenterol. 2008; 103: 2738-2746
        • Bai M.
        • Qi X.
        • Yang Z.
        • Yin Z.
        • Nie Y.
        • Yuan S.
        • et al.
        Predictors of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt in cirrhotic patients: a systematic review.
        J Gastroenterol Hepatol. 2011; 26: 943-951
        • Spina G.
        • Santambrogio R.
        The role of portosystemic shunting in the management of portal hypertension.
        Baillieres Clin Gastroenterol. 1992; 6: 497-515
        • Stepanova M.
        • Mishra A.
        • Venkatesan C.
        • Younossi Z.M.
        In-hospital mortality and economic burden associated with hepatic encephalopathy in the United States from 2005 to 2009.
        Clin Gastroenterol Hepatol. 2012; 10: 1034-1041
        • Kim W.R.
        • Brown Jr., R.S.
        • Terrault N.A.
        • El-Serag H.
        Burden of liver disease in the United States: summary of a workshop.
        Hepatology. 2002; 36: 227-242
        • Fleming K.M.
        • Aithal G.P.
        • Solaymani-Dodaran M.
        • Card T.R.
        • West J.
        Incidence and prevalence of cirrhosis in the United Kingdom, 1992–2001: a general population-based study.
        J Hepatol. 2008; 49: 732-738
        • Gitlin N.
        • Lewis D.C.
        • Hinkley L.
        The diagnosis and prevalence of sub-clinical hepatic encephalopathy in apparently healthy, ambulant, non-shunted patients with cirrhosis.
        J Hepatol. 1986; 3: 75-82
        • Lockwood A.H.
        “What’s in a name?” Improving the care of cirrhotics.
        J Hepatol. 2000; 32: 859-861
        • Amodio P.
        • Montagnese S.
        • Gatta A.
        • Morgan M.Y.
        Characteristics of minimal hepatic encephalopathy.
        Metab Brain Dis. 2004; 19: 253-267
        • McCrea M.
        • Cordoba J.
        • Vessey G.
        • Blei A.T.
        • Randolph C.
        Neuropsycho-logical characterization and detection of subclinical hepatic encephalopathy.
        Arch Neurol. 1996; 53: 758-763
        • Wiltfang J.
        • Nolte W.
        • Weissenborn K.
        • Kornhuber J.
        • Ruther E.
        Psychiatric aspects of portal-systemic encephalopathy.
        Metab Brain Dis. 1998; 13: 379-389
        • Montagnese S.
        • De Pitta C.
        • De Rui M.
        • Corrias M.
        • Turco M.
        • Merkel C.
        • et al.
        Sleep-wake abnormalities in patients with cirrhosis.
        Hepatology. 2014; 59: 705-712
        • Cordoba J.
        • Cabrera J.
        • Lataif L.
        • Penev P.
        • Zee P.
        • Blei A.T.
        High prevalence of sleep disturbance in cirrhosis.
        Hepatology. 1998; 27: 339-345
        • Montagnese S.
        • Middleton B.
        • Skene D.J.
        • Morgan M.Y.
        Night-time sleep disturbance does not correlate with neuropsychiatric impairment in patients with cirrhosis.
        Liver Int. 2009; 29: 1372-1382
        • Weissenborn K.
        Diagnosis of encephalopathy.
        Digestion. 1998; 59: 22-24
        • Adams R.D.
        • Foley J.M.
        The neurological disorder associated with liver disease.
        Res Publ Assoc Res Nerv Ment Dis. 1953; 32: 198-237
        • Cadranel J.F.
        • Lebiez E.
        • Di Martino V.
        • Bernard B.
        • El Koury S.
        • Tourbah A.
        • et al.
        Focal neurological signs in hepatic encephalopathy in cirrhotic patients: an underestimated entity?.
        Am J Gastroenterol. 2001; 96: 515-518
        • Delanty N.
        • French J.A.
        • Labar D.R.
        • Pedley T.A.
        • Rowan A.J.
        Status epilepticus arising de novo in hospitalized patients: an analysis of 41 patients.
        Seizure. 2001; 10: 116-119
        • Eleftheriadis N.
        • Fourla E.
        • Eleftheriadis D.
        • Karlovasitou A.
        Status epilepticus as a manifestation of hepatic encephalopathy.
        Acta Neurol Scand. 2003; 107: 142-144
        • Prabhakar S.
        • Bhatia R.
        Management of agitation and convulsions in hepatic encephalopathy.
        Indian J Gastroenterol. 2003; 22: S54-S58
        • Weissenborn K.
        • Bokemeyer M.
        • Krause J.
        • Ennen J.
        • Ahl B.
        Neurological and neuropsychiatric syndromes associated with liver disease.
        AIDS. 2005; 19: S93-S98
        • Read A.E.
        • Sherlock S.
        • Laidlaw J.
        • Walker J.G.
        The neuro-psychiatric syndromes associated with chronic liver disease and an extensive portal-systemic collateral circulation.
        Q J Med. 1967; 141: 135-150
        • Baccarani U.
        • Zola E.
        • Adani G.L.
        • Cavalletti M.
        • Schiff S.
        • Cagnin A.
        • et al.
        Reversal of hepatic myelopathy after liver transplantation: fifteen plus one.
        Liver Transpl. 2010; 16: 1336-1337
        • Victor M.
        • Adams R.D.
        • Cole M.
        The acquired (non Wilsonian) type of chronic hepatocerebral degeneration.
        Medicine. 1965; 44: 345-396
        • Tryc A.B.
        • Goldbecker A.
        • Berding G.
        • Rümke S.
        • Afshar K.
        • Shahrezaei G.H.
        • et al.
        Cirrhosis-related Parkinsonism: prevalence, mechanisms and response to treatments.
        J Hepatol. 2013; 58: 698-705
        • Lee W.M.
        • Stravitz R.T.
        • Larson A.M.
        Introduction to the revised American Association for the Study of Liver Diseases Position Paper on acute liver failure 2011.
        Hepatology. 2012; 55: 965-967
      1. American Association for the Study of Liver Diseases Position Paper on acute liver failure 2011. Full text. Available at: www.aasld.org/practice-guidelines/Documents/AcuteLiverFailureUpdate2011.pdf.

        • Cordoba J.
        • Ventura-Cots M.
        • Simón-Talero M.
        • Amorós A.
        • Pavesi M.
        • Vilstrup H.
        • et al.
        CANONIC Study Investigators of the EASL-CLIF Consortium. Characteristics, risk factors, and mortality of cirrhotic patients hospitalized for hepatic encephalopathy with and without acute-on-chronic liver failure (ACLF).
        J Hepatol. 2014; 60: 275-281
        • Bajaj J.S.
        • Wade J.B.
        • Sanyal A.J.
        Spectrum of neurocognitive impairment in cirrhosis: implications for the assessment of hepatic encephalopathy.
        Hepatology. 2009; 50: 2014-2021
        • Bajaj J.S.
        • Cordoba J.
        • Mullen K.D.
        • Amodio P.
        • Shawcross D.L.
        • Butterrworth R.F.
        • et al.
        Review article: the design of clinical trials in hepatic encephalopathy—an International Society for Hepatic Encephalopathy and Nitrogen Metabolism (ISHEN) consensus statement.
        Aliment Pharmacol Ther. 2011; 33: 739-747
        • Montagnese S.
        • Amodio P.
        • Morgan M.Y.
        Methods for diagnosing hepatic encephalopathy in patients with cirrhosis: a multidimensional approach.
        Metab Brain Dis. 2004; 19: 281-312
        • Prakash R.
        • Mullen K.D.
        Mechanisms, diagnosis and management of hepatic encephalopathy.
        Nat Rev Gastroenterol Hepatol. 2010; 7: 515-525
        • Hassanein T.I.
        • Hilsabeck R.C.
        • Perry W.
        Introduction to the Hepatic Encephalopathy Scoring Algorithm (HESA).
        Dig Dis Sci. 2008; 53: 529-538
        • Guerit J.M.
        • Amantini A.
        • Fischer C.
        • Kaplan P.W.
        • Mecarelli O.
        • Schnitzler A.
        • et al.
        Neurophysiological investigations of hepatic encephalopathy: ISHEN practice guidelines.
        Liver Int. 2009; 29: 789-796
        • Randolph C.
        • Hilsabeck R.
        • Kato A.
        • Kharbanda P.
        • Li Y.Y.
        • Mapelli D.
        • et al.
        Neuropsychological assessment of hepatic encephalopathy: ISHEN practice guidelines.
        Liver Int. 2009; 29: 629-635
        • Lauridsen M.M.
        • Jepsen P.
        • Vilstrup H.
        Critical flicker frequency and continuous reaction times for the diagnosis of minimal hepatic encephalopathy: a comparative study of 154 patients with liver disease.
        Metab Brain Dis. 2011; 26: 135-139
        • Bajaj J.S.
        • Pinkerton S.D.
        • Sanyal A.J.
        • Heuman D.M.
        Diagnosis and treatment of minimal hepatic encephalopathy to prevent motor vehicle accidents: a cost-effectiveness analysis.
        Hepatology. 2012; 55: 1164-1171
        • Ortiz M.
        • Jacas C.
        • Cordoba J.
        Minimal hepatic encephalopathy: diagnosis, clinical significance and recommendations.
        J Hepatol. 2005; 42: S45-S53
        • Bajaj J.S.
        • Gillevet P.M.
        • Patel N.R.
        • Ahluwalia V.
        • Ridlon J.M.
        • Kettenmann B.
        • et al.
        A longitudinal systems biology analysis of lactulose withdrawal in hepatic encephalopathy.
        Metab Brain Dis. 2012; 27: 205-215
        • Weissenborn K.
        • Ennen J.C.
        • Schomerus H.
        • Ruckert N.
        • Hecker H.
        Neuropsychological characterization of hepatic encephalopathy.
        J Hepatol. 2001; 34: 768-773
        • Prakash R.K.
        • Brown T.A.
        • Mullen K.D.
        Minimal hepatic encephalopathy and driving: is the genie out of the bottle?.
        Am J Gastroenterol. 2011; 106: 1415-1416
        • Bajaj J.S.
        • Stein A.C.
        • Dubinsky R.M.
        What is driving the legal interest in hepatic encephalopathy?.
        Clin Gastroenterol Hepatol. 2011; 9: 97-98
        • Dhiman R.K.
        • Saraswat V.A.
        • Verma M.
        • Naik S.R.
        Figure connection test: a universal test for assessment of mental state.
        J Gastroenterol Hepatol. 1995; 10: 14-23
        • Kircheis G.
        • Wettstein M.
        • Timmermann L.
        • Schnitzler A.
        • Haussinger D.
        Critical flicker frequency for quantification of low-grade hepatic encephalopathy.
        Hepatology. 2002; 35: 357-366
        • Romero-Gomez M.
        • Cordoba J.
        • Jover R.
        • del Olmo J.A.
        • Ramirez M.
        • Rey R.
        • et al.
        Value of the critical flicker frequency in patients with minimal hepatic encephalopathy.
        Hepatology. 2007; 45: 879-885
        • Lauridsen M.M.
        • Thiele M.
        • Kimer N.
        • Vilstrup H.
        The continuous reaction times method for diagnosing, grading, and monitoring minimal/covert hepatic encephalopathy.
        Metab Brain Dis. 2013; 28: 231-234
        • Bajaj J.S.
        • Hafeezullah M.
        • Franco J.
        • Varma R.R.
        • Hoffmann R.G.
        • Knox J.F.
        • et al.
        Inhibitory control test for the diagnosis of minimal hepatic encephalopathy.
        Gastroenterology. 2008; 135: 1591-1600
        • Bajaj J.S.
        • Thacker L.R.
        • Heumann D.M.
        • Fuchs M.
        • Sterling R.K.
        • Sanyal A.J.
        • et al.
        The Stroop smartphone application is a short and valid method to screen for minimal hepatic encephalopathy.
        Hepatology. 2013; 58: 1122-1132
        • Amodio P.
        • Del Piccolo F.
        • Marchetti P.
        • Angeli P.
        • Iemmolo R.
        • Caregaro L.
        • et al.
        Clinical features and survival of cirrhotic patients with sub-clinical cognitive alterations detected by the number connection test and computerized psychometric tests.
        Hepatology. 1999; 29: 1662-1667
        • Montagnese S.
        • Biancardi A.
        • Schiff S.
        • Carraro P.
        • Carla V.
        • Mannaioni G.
        • et al.
        Different biochemical correlates for different neuropsychiatric abnormalities in patients with cirrhosis.
        Hepatology. 2010; 53: 558-566
        • Lockwood A.H.
        Blood ammonia levels and hepatic encephalopathy.
        Metab Brain Dis. 2004; 19: 345-349
        • Grønbaek H.
        • Johnsen S.P.
        • Jepsen P.
        • Gislum M.
        • Vilstrup H.
        • Tage-Jensen U.
        • et al.
        Liver cirrhosis, other liver diseases, and risk of hospitalisation for intracerebral haemorrhage: a Danish population-based case-control study.
        BMC Gastroenterol. 2008; 8: 16
        • Strauss E.
        • Tramote R.
        • Silva E.P.
        • Caly W.R.
        • Honain N.Z.
        • Maffei R.A.
        • et al.
        Double-blind randomized clinical trial comparing neomycin and placebo in the treatment of exogenous hepatic encephalopathy.
        Hepatogastroenterology. 1992; 39: 542-545
        • Als-Nielsen B.
        • Gluud L.L.
        • Gluud C.
        Non-absorbable disaccharides for hepatic encephalopathy: systematic review of randomised trials.
        BMJ. 2004; 328: 1046
        • Riggio O.
        • Varriale M.
        • Testore G.P.
        • Di Rosa R.
        • Di Rosa E.
        • Merli M.
        • et al.
        Effect of lactitol and lactulose administration on the fecal flora in cirrhotic patients.
        J Clin Gastroenterol. 1990; 12: 433-436
        • Huang E.
        • Esrailian E.
        • Spiegel B.M.
        The cost-effectiveness and budget impact of competing therapies in hepatic encephalopathy—a decision analysis.
        Aliment Pharmacol Ther. 2007; 26: 1147-1161
        • Camma C.
        • Fiorello F.
        • Tine F.
        • Marchesini G.
        • Fabbri A.
        • Pagliaro L.
        Lactitol in treatment of chronic hepatic encephalopathy. A meta-analysis.
        Dig Dis Sci. 1993; 38: 916-922
        • Morgan M.Y.
        • Hawley K.E.
        • Stambuk D.
        Lactitol vs. lactulose in the treatment of chronic hepatic encephalopathy. A double-blind, randomised, cross-over study.
        J Hepatol. 1987; 4: 236-244
        • Uribe M.
        • Berthier J.M.
        • Lewis H.
        • Mata J.M.
        • Sierra J.G.
        • García-Ramos G.
        • et al.
        Lactose enemas plus placebo tablets vs. neomycin tablets plus starch enemas in acute portal systemic encephalopathy. A double-blind randomized controlled study.
        Gastroenterology. 1981; 81: 101-106
        • Uribe M.
        • Campollo O.
        • Vargas F.
        • Ravelli G.P.
        • Mundo F.
        • Zapata L.
        • et al.
        Acidifying enemas (lactitol and lactose) vs. nonacidifying enemas (tap water) to treat acute portal-systemic encephalopathy: a double-blind, randomized clinical trial.
        Hepatology. 1987; 7: 639-643
        • Rahimi R.S.
        • Singal A.G.
        • Cuthbert J.A.
        • Rockey D.G.
        A randomized trial of polyethylene glycol 3350-electrolyte solution (PEG) and lactulose for patients hospitalized with acute hepatic encephalopathy.
        Hepatology. 2012; 56 ([abstr. 1546]): 915A-916A
        • Conn H.O.
        • Lieberthal M.M.
        The hepatic coma syndromes and lactulose.
        Williams and Wilkins, Baltimore, MA1979
        • Bajaj J.S.
        • Sanyal A.J.
        • Bell D.
        • Gilles H.
        • Heuman D.M.
        Predictors of the recurrence of hepatic encephalopathy in lactulose-treated patients.
        Aliment Pharmacol Ther. 2010; 31: 1012-1017
        • Patidar K.R.
        • Bajaj J.S.
        Antibiotics for the treatment of hepatic encephalopathy.
        Metab Brain Dis. 2013; 28: 307-312
        • Bass N.M.
        • Mullen K.D.
        • Sanyal A.
        • Poordad F.
        • Neff G.
        • Leevy C.B.
        • et al.
        Rifaximin treatment in hepatic encephalopathy.
        N Engl J Med. 2010; 362: 1071-1081
        • Gluud L.L.
        • Dam G.
        • Borre M.
        • Les I.
        • Cordoba J.
        • Marchesini G.
        • et al.
        Lactulose, rifaximin or branched chain amino acids for hepatic encephalopathy: what is the evidence?.
        Metab Brain Dis. 2013; 28: 221-225
        • Ventura-Cots M.
        • Arranz J.A.
        • Simόn-Talero M.
        • Torrens M.
        • Blanco A.
        • Riudor E.
        • et al.
        Safety of ornithine phenylacetate in cirrhotic decompensated patients: an open-label, dose-escalating, single-cohort study.
        J Clin Gastroenterol. 2013; 47: 881-887
        • Rockey D.C.
        • Vierling J.M.
        • Mantry P.
        • Ghabril M.
        • Brown Jr., R.S.
        • Alexeeva O.
        • et al.
        HALT-HE Study Group. Randomized, double-blind, controlled study of glycerol phenylbutyrate in hepatic encephalopathy.
        Hepatology. 2014; 59: 1073-1083
        • Kircheis G.
        • Nilius R.
        • Held C.
        • Berndt H.
        • Buchner M.
        • Gortelmeyer R.
        • et al.
        Therapeutic efficacy of L-ornithine-L-aspartate infusions in patients with cirrhosis and hepatic encephalopathy: results of a placebo-controlled, double-blind study.
        Hepatology. 1997; 25: 1351-1360
        • Agrawal A.
        • Sharma B.C.
        • Sharma P.
        • Sarin S.K.
        Secondary prophylaxis of hepatic encephalopathy in cirrhosis: an open-label, randomized controlled trial of lactulose, probiotics, and no therapy.
        Am J Gastroenterol. 2012; 107: 1043-1050
        • Hawkins R.A.
        • Jessy J.
        • Mans A.M.
        • Chedid A.
        • DeJoseph M.R.
        Neomycin reduces the intestinal production of ammonia from glutamine.
        Adv Exp Med Biol. 1994; 368: 125-134
        • Morgan M.H.
        • Read A.E.
        • Speller D.C.
        Treatment of hepatic encephalopathy with metronidazole.
        Gut. 1982; 23: 1-7
        • Simón-Talero M.
        • García-Martínez R.
        • Torrens M.
        • Augustin S.
        • Gómez S.
        • Pereira G.
        • et al.
        Effects of intravenous albumin in patients with cirrhosis and episodic hepatic encephalopathy: a randomized double-blind study.
        J Hepatol. 2013; 59: 1184-1192
        • Sharma P.
        • Agrawal A.
        • Sharma B.C.
        • Sarin S.K.
        Prophylaxis of hepatic encephalopathy in acute variceal bleed: a randomized controlled trial of lactulose vs. no lactulose.
        J Gastroenterol Hepatol. 2011; 26: 996-1003
        • Riggio O.
        • Masini A.
        • Efrati C.
        • Nicolao F.
        • Angeloni S.
        • Salvatori F.M.
        • et al.
        Pharmacological prophylaxis of hepatic encephalopathy after transjugular intrahepatic portosystemic shunt: a randomized controlled study.
        J Hepatol. 2005; 42: 674-679
        • Fanelli F.
        • Salvatori F.M.
        • Rabuffi P.
        • Boatta E.
        • Riggio O.
        • Lucatelli P.
        • et al.
        Management of refractory hepatic encephalopathy after insertion of TIPS: long-term results of shunt reduction with hourglass-shaped balloon-expandable stent-graft.
        AJR Am J Roentgenol. 2009; 193: 1696-1702
        • Chung H.H.
        • Razavi M.K.
        • Sze D.Y.
        • Frisoli J.K.
        • Kee S.T.
        • Dake M.D.
        • et al.
        Portosystemic pressure gradient during transjugular intrahepatic portosystemic shunt with Viatorr stent graft: what is the critical low threshold to avoid medically uncontrolled low pressure gradient related complications?.
        J Gastroenterol Hepatol. 2008; 23: 95-101
        • Laleman W.
        • Simon-Talero M.
        • Maleux G.
        • Perez M.
        • Ameloot K.
        • Soriano G.
        • et al.
        • on behalf of the EASL-CLIF-Consortium
        Embolization of large spontaneous portosystemic shunts for refractory hepatic encephalopathy: a multi-center survey on safety and efficacy.
        Hepatology. 2013; 57: 2448-2457
        • Sharma P.
        • Sharma B.C.
        • Agrawal A.
        • Sarin S.K.
        Primary prophylaxis of overt hepatic encephalopathy in patients with cirrhosis: an open labeled randomized controlled trial of lactulose vs. no lactulose.
        J Gastroenterol Hepatol. 2012; 27: 1329-1335
        • Sidhu S.S.
        • Goyal O.
        • Mishra B.P.
        • Sood A.
        • Chhina R.S.
        • Soni R.K.
        Rifaximin improves psychometric performance and health-related quality of life in patients with minimal hepatic encephalopathy (the RIME Trial).
        Am J Gastroenterol. 2011; 106: 307-316
        • Bajaj J.S.
        • Heuman D.M.
        • Wade J.B.
        • Gibson D.P.
        • Saeian K.
        • Wegelin J.A.
        • et al.
        Rifaximin improves driving simulator performance in a randomized trial of patients with minimal hepatic encephalopathy.
        Gastroenterology. 2011; 140: 478-487
        • Bajaj J.S.
        • Saeian K.
        • Christensen K.M.
        • Hafeezullah M.
        • Varma R.R.
        • Franco J.
        • et al.
        Probiotic yogurt for the treatment of minimal hepatic encephalopathy.
        Am J Gastroenterol. 2008; 103: 1707-1715
        • Mittal V.V.
        • Sharma B.C.
        • Sharma P.
        • Sarin S.K.
        A randomized controlled trial comparing lactulose, probiotics, and L-ornithine L-aspartate in treatment of minimal hepatic encephalopathy.
        Eur J Gastroenterol Hepatol. 2011; 23: 725-732
        • Saji S.
        • Kumar S.
        • Thomas V.
        A randomized double blind placebo controlled trial of probiotics in minimal hepatic encephalopathy.
        Trop Gastroenterol. 2011; 32: 128-132
        • Shukla S.
        • Shukla A.
        • Mehboob S.
        • Guha S.
        Meta-analysis: the effects of gut flora modulation using prebiotics, probiotics and synbiotics on minimal hepatic encephalopathy.
        Aliment Pharmacol Ther. 2011; 33: 662-671
        • Amodio P.
        • Bemeur C.
        • Butterworth R.
        • Cordoba J.
        • Kata A.
        • Montagnese S.
        • et al.
        The nutritional management of hepatic encephalopathy in patients with cirrhosis: ISHEN practice guidelines.
        Hepatology. 2013; 58: 325-336
        • Montano-Loza A.J.
        • Meza-Junco J.
        • Prado C.M.
        • Lieffers J.R.
        • Baracos V.E.
        • Bain V.G.
        • et al.
        Muscle wasting is associated with mortality in patients with cirrhosis.
        Clin Gastroenterol Hepatol. 2012; 10: 166-173
        • Tandon P.
        • Ney M.
        • Irwin I.
        • Ma M.M.
        • Gramlich L.
        • Bain V.G.
        • et al.
        Severe muscle depletion in patients on the liver transplant wait list: its prevalence and independent prognostic value.
        Liver Transpl. 2012; 18: 1209-1216
        • Tsien C.D.
        • McCullough A.J.
        • Dasarathy S.
        Late evening snack: exploiting a period of anabolic opportunity in cirrhosis.
        J Gastroenterol Hepatol. 2012; 27: 430-441
        • Ndraha S.
        • Hasan I.
        • Simadibrata M.
        The effect of L-ornithine L-aspartate and branch chain amino acids on encephalopathy and nutritional status in liver cirrhosis with malnutrition.
        Acta Med Indones. 2011; 43: 18-22
        • Naylor C.D.
        • O’Rourke K.
        • Detsky A.S.
        • Baker J.P.
        Parenteral nutrition with branched-chain amino acids in hepatic encephalopathy. A meta-analysis.
        Gastroenterology. 1989; 97: 1033-1042
        • Marchesini G.
        • Bianchi G.
        • Merli M.
        • Amodio P.
        • Panella C.
        • Loguercio C.
        • et al.
        Nutritional supplementation with branched-chain amino acids in advanced cirrhosis: a double-blind, randomized trial.
        Gastroenterology. 2003; 124: 1792-1801
        • Marchesini G.
        • Marzocchi R.
        • Noia M.
        • Bianchi G.
        Branched-chain amino acid supplementation in patients with liver diseases.
        J Nutr. 2005; 135: 1596S-1601S
        • Gluud L.L.
        • Dam G.
        • Borre M.
        • Les I.
        • Cordoba J.
        • Marchesini G.
        • et al.
        Oral branched chain amino acids have a beneficial effect on manifestations of hepatic encephalopathy in a systematic review with meta-analyses of randomized controlled trials.
        J Nutr. 2013; 143: 1263-1268
        • Martin P.
        • DiMartini A.
        • Feng S.
        • Brown Jr., R.
        • Fallon M.
        Evaluation for liver transplantation in adults: 2013 Practice Guideline by the American Association for the Study of Liver Diseases and the American Society of Transplantation.
        Hepatology. 2014; 59: 1144-1165
        • Lucey M.R.
        • Terrault N.
        • Ojo L.
        • Hay J.E.
        • Neuberger J.
        • Blumberg E.
        • et al.
        Long-term management of the successful adult liver transplant: 2012 Practice Guideline by the American Association for the Study of Liver Diseases and the American Society of Transplantation.
        Liver Transpl. 2013; 19: 3-26
        • Herrero J.I.
        • Bilbao J.I.
        • Diaz M.L.
        • Alegre F.
        • Inarrairaegui M.
        • Pardo F.
        • et al.
        Hepatic encephalopathy after liver transplantation in a patient with a normally functioning graft: treatment with embolization of portosystemic collaterals.
        Liver Transpl. 2009; 15: 111-114
        • Chavarria L.
        • Alonso J.
        • García-Martínez R.
        • Simón-Talero M.
        • Ventura-Cots M.
        • Ramírez C.
        • et al.
        Brain magnetic resonance spectroscopy in episodic hepatic encephalopathy.
        J Cereb Blood Flow Metab. 2013; 33: 272-277
        • Garcia-Martinez R.
        • Rovira A.
        • Alonso J.
        • Jacas C.
        • Simón-Talero M.
        • Chavarria L.
        • et al.
        Hepatic encephalopathy is associated with posttransplant cognitive function and brain volume.
        Liver Transpl. 2011; 17: 38-46
        • Amodio P.
        • Biancardi A.
        • Montagnese S.
        • Angeli P.
        • Iannizzi P.
        • Cillo U.
        • et al.
        Neurological complications after orthotopic liver transplantation.
        Dig Liver Dis. 2007; 39: 740-747
        • Poordad F.F.
        Review article: the burden of hepatic encephalopathy.
        Aliment Pharmacol Ther. 2007; 25: 3-9
        • Bajaj J.S.
        • Wade J.B.
        • Gibson D.P.
        • Heuman D.M.
        • Thacker L.R.
        • Sterling R.K.
        • et al.
        The multi-dimensional burden of cirrhosis and hepatic encephalopathy on patients and caregivers.
        Am J Gastroenterol. 2011; 106: 1646-1653
        • Neff G.W.
        • Kemmer N.
        • Zacharias V.C.
        • Kaiser T.
        • Duncan C.
        • McHenry R.
        • et al.
        Analysis of hospitalizations comparing rifaximin vs. lactulose in the management of hepatic encephalopathy.
        Transplant Proc. 2006; 38: 3552-3555
        • Guevara M.
        • Baccaro M.E.
        • Torre A.
        • Gómez-Ansón B.
        • Rìos J.
        • Torres F.
        • et al.
        Hyponatremia is a risk factor of hepatic encephalopathy in patients with cirrhosis: a prospective study with time-dependent analysis.
        Am J Gastroenterol. 2009; 104: 1382-1389
        • Guevara M.
        • Baccaro M.E.
        • Ríos J.
        • Martín-Llahí M.
        • Uriz J.
        • Ruiz del Arbol L.
        • et al.
        Risk factors for hepatic encephalopathy in patients with cirrhosis and refractory ascites: relevance of serum sodium concentration.
        Liver Int. 2010; 30: 1137-1142
        • Angeli P.
        • Wong F.
        • Watson H.
        • Gines P.
        Hyponatremia in cirrhosis: results of a patient population survey.
        Hepatology. 2006; 44: 1535-1542
        • Sharma P.
        • Sharma B.C.
        • Sarin S.K.
        Predictors of nonresponse to lactulose for minimal hepatic encephalopathy in patients with cirrhosis.
        Liver Int. 2009; 29: 1365-1371
        • Sigal S.H.
        • Stanca C.M.
        • Kontorinis N.
        • Bodian C.
        • Ryan E.
        Diabetes mellitus is associated with hepatic encephalopathy in patients with HCV cirrhosis.
        Am J Gastroenterol. 2006; 101: 1490-1496
        • Kalaitzakis E.
        • Olsson R.
        • Henfridsson P.
        • Hugosson I.
        • Bengtsson M.
        • Jalan R.
        • et al.
        Malnutrition and diabetes mellitus are related to hepatic encephalopathy in patients with liver cirrhosis.
        Liver Int. 2007; 27: 1194-1201
        • Kalaitzakis E.
        • Bjornsson E.
        Renal function and cognitive impairment in patients with liver cirrhosis.
        Scand J Gastroenterol. 2007; 42: 1238-1244
        • Gustot T.
        • Durand F.
        • Lebrec D.
        • Vincent J.L.
        • Moreau R.
        Severe sepsis in cirrhosis.
        Hepatology. 2009; 50: 2022-2032
        • Young G.B.
        • Bolton C.F.
        • Austin T.W.
        • Archibald Y.M.
        • Gonder J.
        • Wells G.A.
        The encephalopathy associated with septic illness.
        Clin Invest Med. 1990; 13: 297-304
        • Butterworth R.F.
        Thiamine deficiency-related brain dysfunction in chronic liver failure.
        Metab Brain Dis. 2009; 24: 189-196
        • Pitel A.L.
        • Beaunieux H.
        • Witkowski T.
        • Vabret F.
        • Guillery-Girard B.
        • Quinette P.
        • et al.
        Genuine episodic memory deficits and executive dysfunctions in alcoholic subjects early in abstinence.
        Alcohol Clin Exp Res. 2007; 31: 1169-1178
        • Noel X.
        • Van der Linden M.
        • Schmidt N.
        • Sferrazza R.
        • Hanak C.
        • Le Bon O.
        • et al.
        Supervisory attentional system in nonamnestic alcoholic men.
        Arch Gen Psychiatry. 2001; 58: 1152-1158