- •Studying HBV has been limited by the availability of in vitro and in vivo models.
- •A selected HepG2 cell clone expressing NTCP supports long-term HBV infection.
- •HBV has slow infection kinetics requiring 3 days for full establishment of infection.
- •HBV establishes 1–9 copies of cccDNA per cell which have an estimated half-life of 40 days.
- •cccDNA levels remain stable by intracellular genome recycling and secondary infection.
Background & Aims
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Author names in bold designate shared co-first authorship
- Molecular biology of hepatitis B virus infection.Virology. 2015; 479–480C: 672-686
- Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycans.Hepatology. 2007; 46: 1759-1768
- A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses.Hepatology. 2016; 63: 35-48
- Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus.eLife. 2012; 1: e00049
- Hepatitis B and D viruses exploit sodium taurocholate co-transporting polypeptide for species-specific entry into hepatocytes.Gastroenterology. 2014; 146: 1070-1083
- HBV cccDNA: viral persistence reservoir and key obstacle for a cure of chronic hepatitis B.Gut. 2015; 64: 1972-1984
- Involvement of host cellular multivesicular body functions in hepatitis B virus budding.Proc Natl Acad Sci USA. 2007; 104: 10205-10210
- Formation of the pool of covalently closed circular viral DNA in hepadnavirus-infected cells.Cell. 1986; 47: 451-460
- Generation of covalently closed circular DNA of hepatitis B viruses via intracellular recycling is regulated in a virus specific manner.PLoS Pathog. 2010; 6: e1001082
- HBV core protein allosteric modulators differentially alter cccDNA biosynthesis from de novo infection and intracellular amplification pathways.PLoS Pathog. 2017; 13: e1006658
- Avoiding the void: cell-to-cell spread of human viruses.Nat Rev Microbiol. 2008; 6: 815-826
- Hepatitis C virus cell-cell transmission in hepatoma cells in the presence of neutralizing antibodies.Hepatology. 2008; 47: 17-24
- Analyses of HBV cccDNA quantification and modification.Methods Mol Biol. 2017; 1540: 59-72
- Identification of hydrolyzable tannins (punicalagin, punicalin and geraniin) as novel inhibitors of hepatitis B virus covalently closed circular DNA.Antiviral Res. 2016; 134: 97-107
- Residues Arg703, Asp777, and Arg781 of the RNase H domain of hepatitis B virus polymerase are critical for viral DNA synthesis.J Virol. 2014; 88: 154-163
- N-Glycosylation of the Na+-taurocholate cotransporting polypeptide (NTCP) determines its trafficking and stability and is required for hepatitis b virus infection.PLoS ONE. 2017; 12: e0170419
- Reproducible high level infection of cultured adult human hepatocytes by hepatitis B virus: effect of polyethylene glycol on adsorption and penetration.Virology. 1993; 192: 534-540
- Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis B virus: an intermediate of covalently closed circular DNA formation.J Virol. 2007; 81: 12472-12484
- Formation of hepatitis B virus covalently closed circular DNA: removal of genome-linked protein.J Virol. 2007; 81: 6164-6174
- Optimizing human hepatocyte models for metabolic phenotype and function: effects of treatment with dimethyl sulfoxide (DMSO).Physiol Rep. 2016; 4
- Proliferation of primary human hepatocytes and prevention of hepatitis B virus reinfection efficiently deplete nuclear cccDNA in vivo.Gut. 2018; 67: 542-552
- A robust cell culture system supporting the complete life cycle of hepatitis B virus.Sci Rep. 2017; 7: 16616
- Transfer of hepatitis B virus genome by adenovirus vectors into cultured cells and mice: crossing the species barrier.J Virol. 2001; 75: 5108-5118
- Human stem cell-derived hepatocytes as a model for hepatitis B virus infection, spreading and virus-host interactions.J Hepatol. 2017; 66: 494-503
- Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis.J Virol. 2001; 75: 311-322
- Half-life of the duck hepatitis B virus covalently closed circular DNA pool in vivo following inhibition of viral replication.J Virol. 2002; 76: 6356-6363
- CD8(+) T cells mediate viral clearance and disease pathogenesis during acute hepatitis B virus infection.J Virol. 2003; 77: 68-76
- The entry inhibitor Myrcludex-B efficiently blocks intrahepatic virus spreading in humanized mice previously infected with hepatitis B virus.J Hepatol. 2013; 58: 861-867
- Evidence that hepatitis B virus replication in mouse cells is limited by the lack of a host cell dependency factor.J Hepatol. 2016; 64: 556-564
- Morphogenetic and regulatory effects of mutations in the envelope proteins of an avian hepadnavirus.J Virol. 1991; 65: 1310-1317